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Series GSE35952 Query DataSets for GSE35952
Status Public on Mar 08, 2012
Title Global Mapping at the Core of the Arabidopsis Circadian Clock Defines the Network Structure of the Oscillator
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In many organisms, the circadian clock is composed of functionally coupled morning and evening oscillators that regulate the bouts of dawn and dusk activity. In Arabidopsis, oscillator coupling relies on a core loop in which the evening oscillator component TOC1 was proposed to activate a subset of morning-expressed oscillator genes. Our systems-biological approach overturns the current view of the Arabidopsis circadian clock showing that TOC1 does not function as an activator but as a timely-controlled general repressor of morning and evening oscillator components. Repression occurs through rhythmic binding to the promoters of all oscillator genes, suggesting a previously unexpected direct connection between the morning and evening loops.
Overall design Examination of TOC1 genome-wide binding using TOC1 Minigene (TMG) seedlings expressing the genomic fragment of TOC1 fused to the Yellow Fluorescent Protein in a toc1-2 mutant background (TMG-YFP/toc1-2 seedlings) grown under LD cycles (12h light:12h dark).
Contributor(s) Riechmann JL, Antoshechkin I, Mas P
Citation(s) 22403178
Submission date Feb 21, 2012
Last update date Sep 04, 2019
Contact name Jose Luis Riechmann
Organization name Center for Research in Agricultural Genomics
Street address Campus UAB - Edifici CRAG
City Bellaterra
State/province Barcelona
ZIP/Postal code 08193
Country Spain
Platforms (1)
GPL11221 Illumina Genome Analyzer IIx (Arabidopsis thaliana)
Samples (2)
GSM878068 TOC1_ChIPSeq
GSM878069 input DNA
SRA SRP010999
BioProject PRJNA151987

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Supplementary file Size Download File type/resource
GSE35952_RAW.tar 522.1 Mb (http)(custom) TAR (of BED, TXT)
GSE35952_readme.txt.gz 689 b (ftp)(http) TXT
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Processed data provided as supplementary file
Raw data are available in SRA

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