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| Status |
Public on Aug 15, 2012 |
| Title |
Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Studies of adult human hematopoiesis have until now relied on the expression of CD10 to define lymphoid commitment. We report a novel lymphoid-primed population in human bone marrow that is generated from hematopoietic stem cells (HSC) prior to the onset of CD10 expression and B cell commitment, and is identified by high levels of the homing molecule L-selectin (CD62L). CD10-CD62Lhi progenitors have full lymphoid (B/T/NK) potential, and show reduced myeloid and absent erythroid potential. Genome-wide gene expression analysis demonstrates that the CD10-CD62Lhi population represents an intermediate stage of differentiation between CD34+CD38- HSC and CD34+lin-CD10+ progenitors marked by down-regulation of TAL1 and MPL, upregulation of E2A, CD3E and IL2RG expression, and absent B cell commitment or RAG1/2 expression. Immature CD34+CD1a- thymocytes are also CD62Lhi and L-selectin ligands are expressed at the cortico-medullary junction, suggesting a possible role for L-selectin in human thymic homing. These studies identify the earliest stage of lymphoid priming in human bone marrow.
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| Overall design |
Freshly harvested human bone marrow was ficoll purified, enriched for CD34+, and then FACS sorted. It was then sorted into 3 samples CD34+CD38-, CD34+ CD10- CD62L++, and CD34+ CD10+, with 3 independent biological replicates
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| Contributor(s) |
Kohn LA, Hao Q, Sasidharan R, Parekh C, Ge S, Zhu J, Mikkola H, Crooks GM |
| Citation(s) |
22941246 |
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| Submission date |
Feb 10, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Lisa Kohn |
| E-mail(s) |
Lakohn@ucla.edu
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| Organization name |
UCLA
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| Street address |
650 charles e young drive south
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| City |
los angeles |
| State/province |
ca |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA152411 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE35685_RAW.tar |
39.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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