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Series GSE35395 Query DataSets for GSE35395
Status Public on Jul 03, 2012
Title Expression from early pre-hematopoietic progenitors from mouse embryo
Organism Mus musculus
Experiment type Expression profiling by array
Summary Hematopoietic Stem Cells (HSC) are originated during embryonic development from endothelial-like cells located in the ventral side of the dorsal aorta around day E10-12 of murine development. This region is called AGM for Aorta/Gonad/Mesonephros and refers to the tissues around the hemogenic aorta. Cells that emerge from the endothelium and show hematopoietic traits can be distinguished by the expression of the c-kit receptor and finally acquire the CD45 marker.
 
Overall design AGM regions were obtained from E11.5 embryos by dissection and digested with 0.1% collagenase. Cells were stained with anti-CD31, anti-ckit, anti-CD45 and anti-Ter119 antibodies. Sorting of the CD31+CD45-Ter119- population was performed, and cells were separated into c-kit+ and c-kit-. 3 replicates each of c-kit+ and c-kit- cells.
 
Contributor(s) Bigas A, Guiu J, Ruiz-Herguido C, Espinosa L
Citation(s) 22802352
Submission date Jan 27, 2012
Last update date Mar 04, 2019
Contact name Anna Bigas
E-mail abigas@imim.es
Phone +34933160440
Organization name Institut Hospital del Mar d'Investigacions Mèdiques
Department Cancer Research
Lab Stem Cells and Cancer
Street address Dr. Aiguader 88
City Barcelona
State/province Barcelona
ZIP/Postal code 08003
Country Spain
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (6)
GSM867295 Ckit-negative (Ckit-) cells at time T0, biological rep1
GSM867296 Ckit-negative (Ckit-) cells at time T0, biological rep2
GSM867297 Ckit-negative (Ckit-) cells at time T0, biological rep3
Relations
BioProject PRJNA152701

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Supplementary file Size Download File type/resource
GSE35395_RAW.tar 22.7 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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