|
| Status |
Public on Mar 01, 2012 |
| Title |
Deep-sequencing influences the results obtained in small-RNA sequencing |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
This study presents a comparison of small RNA sequencing libraries generated from the same cell lines but using different sequencing platforms and protocols. The samples were analyzed and compared at the level of miRNAs expression and as a population of small RNAs derived from repetitive elements. Despite a good correlation between sequencing platforms, there are qualitative and quantitative variations in the results depending on the protocol used.
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| |
|
| Overall design |
10 samples were examined: 6 from the ES E14 XY cell type: 1 454, 2 SOLiD from 2 technological versions, and 3 SOLEXA from 3 different protocols, and 4 samples from ES PGK XX cells: 1 454 and 1 SOLEXA sample, and 2 SOLiD samples from 2 technological versions.
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| |
|
| Contributor(s) |
Toedling J, Servant N, Ciaudo C, Farinelli L, Voinnet O, Heard E, Barillot E |
| Citation(s) |
22384282 |
| |
| Submission date |
Jan 26, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicolas Servant |
| E-mail(s) |
Nicolas.Servant@curie.fr
|
| Organization name |
Institut Curie
|
| Street address |
26 rue d'ulm
|
| City |
Paris Cedex 05 |
| ZIP/Postal code |
75248 |
| Country |
France |
| |
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| Platforms (5)
|
| GPL9273 |
454 GS FLX (Mus musculus) |
| GPL9318 |
AB SOLiD System 3.0 (Mus musculus) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL14602 |
AB SOLiD 4 System (Mus musculus) |
|
| Samples (10)
|
|
| Relations |
| SRA |
SRP010603 |
| BioProject |
PRJNA152595 |
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