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Series GSE35337 Query DataSets for GSE35337
Status Public on Nov 30, 2012
Title Direct measurement of heritable gene expression within single individuals from wild populations.
Platform organisms Picea glauca; Picea sitchensis
Sample organism Picea glauca
Experiment type Expression profiling by array
Summary Heritable variation in gene expression has been largely studied in model organisms by taking advantage of pure and inbred lines. Comparative studies on organisms with contrasting evolutionary history, population size and genome architecture have been hindered by practical considerations, as many of these species are poorly suited for breeding. We have developed a method for determining expression variation in an undomesticated tree species by directly measuring the segregation of gene expression in the haploid meiotic products of a single diploid individual. We show the utility of our approach by identifying expression variation that segregates consistent with one causal variant and identify an unexpectedly large number of these Mendelian expression traits. The megagametophyte approach opens the doors for the application of genetical genomics approaches in a large number of undomesticated plant species.
Overall design Transcriptomics data from 18 megagametophytes of two unrelated mother trees. In tree 80112, all of the 18 megagametophytes were analyzed on to different microarrays with two different dyes. In tree 77111, 16 megagametophytes were replicated as above.
Contributor(s) Verta J, Caron S, Giguère I, MacKay JJ
Citation(s) 23294325
Submission date Jan 25, 2012
Last update date Jun 18, 2013
Contact name Jukka-Pekka Verta
Organization name Centre for Forest Research
Department Department of Wood and Forest Sciences
Lab John J. MacKay
Street address 1030 Avenue de la Medecine, Pavillon Charles-Eugene-Marchand, bureau 2225
City Quebec
State/province QC
ZIP/Postal code G1V 0A6
Country Canada
Platforms (1)
GPL15033 Arborea spruce 32K
Samples (72)
GSM866241 77111_megagametophyte#56_635
GSM866242 77111_megagametophyte#67_635
GSM866243 77111_megagametophyte#51_635
BioProject PRJNA152705

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35337_RAW.tar 88.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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