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| Status |
Public on Oct 29, 2013 |
| Title |
Transcription profile of adipose derived mesenchymal stem cells separated by cell surface protein N-cadherin. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Mesenchymal stem cells (MSCs) are one of most promising stem cell sources for regenerative medicine. MSCs have a differention ability into several tissues. However, their differentiation efficiency is considered quite limited. Especially, the differentiation efficiency into cardiomyocytes is very low. N-cadherin is one of the cell surface protein that is expressed in developing and adult cardiomyocytes. We found that cell surface expression of N-cadherin in MSCs shows good correlation with the cardiomyogenic differentiation ability. We also analyzed the expression profiles of N-cadherin-positive and N-cadherin-negative fraction by microarray. We found that N-cadherin-positive MSCs show higher expression of some of the cardiomyogenesis-related genes than N-cadherin-negative MSCs.
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| Overall design |
The ASCs were maintained in MesenPRO RSTM Medium. The ASCs were harvested in a cell dissociation buffer. The ASCs were incubated with biotinylated anti N-cadherin antibody and next incubated with streptavidin –APC. Subsequently cells were incubated with anti-APC microbeads. Immunomagnetic separation of N-cadherin+ cells were performed with autoMACSTMPro separator applying the separation program ‘depl025’
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| Contributor(s) |
Ishimine H, Kurisaki A, Asashima M |
| Citation(s) |
23899519 |
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| Submission date |
Jan 20, 2012 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Hisako Ishimine |
| Organization name |
National Institute of Advanced Industrial Science and Technology
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| Department |
Research Center for Stem Cell Engineering
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| Street address |
higashi1-1-1 Tsukuba central 4
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| City |
Tsukuba |
| State/province |
Japan Ibaraki |
| ZIP/Postal code |
305-8562 |
| Country |
Japan |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA152859 |
