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Series GSE35197 Query DataSets for GSE35197
Status Public on Mar 23, 2012
Title A protective strategy against hyperinflammatory responses requiring the non-transcriptional actions of GPS2
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary The association between hyper-inflammatory states and numerous diseases is widely recognized, but our understanding of the molecular strategies that have evolved to prevent uncontrolled activation of inflammatory responses remains incomplete. Here, we report a critical, non-transcriptional role of GPS2 as a guardian against hyperstimulation of TNFA-induced gene program. GPS2 cytoplasmic actions are required to specifically modulate RIP1 ubiquitylation and JNK activation by inhibiting TRAF2/Ubc13 enzymatic activity. In vivo relevance of GPS2 anti-inflammatory role is confirmed by inhibition of TNFA target genes in macrophages and by improved insulin signaling in the adipose tissue of aP2-GPS2 transgenic mice. As the non-transcriptional role is complemented by GPS2 functioning as positive and negative cofactor for nuclear receptors, in vivo overexpression also results in elevated circulating level of resistin and development of hepatic steatosis. Together, these studies define GPS2 as a molecular guardian required for precise control of inflammatory responses involved in immunity and homeostasis.
Overall design RNA-sequencing of polyA selected RNA molecules in 293T cells and ChIP-seq of GPS2, TBL1, and NCOR.
Contributor(s) Perissi V, Cardamone D, Krones A, Tanasa B, Ricci L, Ohgi KH, Glass CK, Rosenfeld MG
Citation(s) 22424771
Submission date Jan 18, 2012
Last update date May 15, 2019
Contact name Valentina Perissi
Phone 617 638 4115
Fax 617 638 5339
Organization name Boston University School of Medicine
Department Department of Biochemistry
Street address 72 E. Concord St
City Boston
State/province MA
ZIP/Postal code 02118
Country USA
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (8)
GSM863382 minusTNFa_siCTL
GSM863383 minusTNFa_siGPS2
GSM863384 plusTNFa_siCTL
SRA SRP010430
BioProject PRJNA150685

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Supplementary file Size Download File type/resource
GSE35197_RAW.tar 2.9 Gb (http)(custom) TAR (of BED, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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