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Series GSE35190 Query DataSets for GSE35190
Status Public on Jan 21, 2012
Title aCGH study of iPS cells from two normal subjects and two Parkinson's disease patients with parkin mutations
Organism Homo sapiens
Experiment type Genome variation profiling by genome tiling array
Summary Parkinson's disease (PD) is defined by the degeneration of nigral dopaminergic (DA) neurons and can be caused by monogenic mutations of genes such as parkin. The lack of phenotype in parkin knockout mice suggests that human nigral DA neurons have unique vulnerabilities. Here we generate induced pluripotent stem cells from normal subjects and PD patients with parkin mutations. We demonstrate that loss of parkin in human midbrain DA neurons greatly increases the transcription of monoamine oxidases and oxidative stress, significantly reduces DA uptake and increases spontaneous DA release. Lentiviral expression of parkin, but not its PD-linked mutant, rescues these phenotypes. The results suggest that parkin controls dopamine utilization in human midbrain DA neurons by enhancing the precision of DA neurotransmission and suppressing dopamine oxidation. Thus, the study provides novel targets and a physiologically relevant screening platform for disease-modifying therapies of PD.
Overall design Genomic DNA was isolated from each of the four lines of iPSCs and labeled with Cy5. Pooled sex mismatched normal human genomic DNA was labeled with Cy3. Both samples are hybridized together on RPCI 21K BAC aCGH array.
Contributor(s) Jiang H, Ren Y, Yuen EY, Zhong P, Ghaedi M, Hu Z, Azabdaftari G, Nakaso K, Yan Z, Feng J
Citation(s) 22314364
Submission date Jan 18, 2012
Last update date Apr 23, 2012
Contact name Jian Feng
Phone 716-829-2345
Organization name State University of New York at Buffalo
Department Department of Physiology and Biophysics
Lab Feng Lab
Street address 124 Sherman Hall
City Buffalo
State/province NY
ZIP/Postal code 14214
Country USA
Platforms (1)
GPL15098 RPCI Human HB21K
Samples (4)
GSM864242 Parkinson patient 1 with parkin mutations
GSM864243 Parkinson patient 2 with parkin mutations
GSM864244 Normal subject 1
BioProject PRJNA150681

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35190_RAW.tar 17.6 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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