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Series GSE35027 Query DataSets for GSE35027
Status Public on Aug 23, 2012
Title Global gene expression analysis of human embryonic stem cells, adult fibroblasts , and CD34+ cord blood (CB) cells before, during, and afer their episomal induction of pluripotency
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Global gene expression analysis of (a) human embryonic stem cells, (b) adult fibroblasts with and without nucleofection of SOKM, and ( c ) CD34+ cord blood cells at various time points during induction of pluripotency with SOKM, with or without co-culture with bone marrow stromal cells (BMSC).
Overall design Total RNA was harvested from adult fibroblasts, adult fibroblasts three days after nucleofection with 4F (SOKM), Day -3 unstimulated cord blood (CB) cells, Day 0 growth factor stimulated CB cells, Day +3 CB cells with or without nucleofection at Day zero and with or without Day zero to Day +3 co-culture with bone marrow stromal cells (BMSC), Day 23 CB cells nucleofected on day zero and co-cultured on BMSC from Day Zero to Day +3, and undifferentiated H9 human embryonic stem cells. All experimental conditions repeated in three independent trials, and each replicate at each condition analyzed on an individual microarray.
Contributor(s) Park TS, Huo JS, Peters A, Talbot CC, Kaplan IM, Zambidis ET
Citation(s) 22905176
Submission date Jan 11, 2012
Last update date Aug 13, 2018
Contact name Elias Thomas Zambidis
Organization name Johns Hopkins School of Medicine
Department Oncology
Street address 733 N. Broadway
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (30)
GSM860961 H9 p52 hESC
GSM860962 H9 p51 hESC
GSM860963 H9 p54 hESC
This SubSeries is part of SuperSeries:
GSE35029 Growth factor-activated stem cell circuits and stromal signals cooperatively accelerate iPSC reprogramming of lineage-committed myeloid progenitors
BioProject PRJNA156183

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35027_RAW.tar 26.2 Mb (http)(custom) TAR
GSE35027_non-normalized.txt.gz 16.1 Mb (ftp)(http) TXT
GSE35027_normalized.txt.gz 4.0 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data are available on Series record
Raw data included within Sample table

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