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Series GSE35005 Query DataSets for GSE35005
Status Public on Mar 12, 2012
Title Epigenetic dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Studies on the epigenetic regulation of gene expression during germ cell development are still at the beginning stage. In the present study, we used our highly specific 5hmC-labeling and enrichment technique coupled with DNA deep-sequencing to profile the global 5hmC distribution in 8 serial stages of male germ cells during spermatogenesis, as well as in the the Sertoli cells (SE) which are the only somatic cell type inside seminiferous tubules. We analyzed the genomic distribution and dynamic changes of 5hmC during spermatogenesis. Moreover, to dissect the functional significance of 5hmC modifications for transcriptional regulation of gene expression, we also performed RNA-Seq transcriptome analysis in all of the 8 corresponding stages of male germ cells and found that 5hmC is positively correlated with gene expression.
Overall design RNA-seq: Examination of the transcriptomes during mouse spermatogenesis.
5hmC-seq: Identification of 5hmC enriched genmoic regions in mouse germ cell.
Contributor(s) Lu W, Tang F, Han C, Liao S, Gan H
Citation(s) 23759713
Submission date Jan 11, 2012
Last update date May 15, 2019
Contact name Chunsheng Han
Phone 86-10-64807105
Organization name Chinese Academy of Sciences
Department Institute of Zoology
Lab State Key Laboratory of Reproductive Biology
Street address 1-5 Beichen West Road, Chaoyang District
City Beijing
State/province Beijing
ZIP/Postal code 100101
Country China
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (18)
GSM860181 priSG-A_RNAseq
GSM860182 SG-A_RNAseq
GSM860183 SG-B_RNAseq
SRA SRP010262
BioProject PRJNA151173

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Supplementary file Size Download File type/resource
GSE35005_RAW.tar 17.0 Gb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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