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| Status |
Public on Mar 12, 2012 |
| Title |
Epigenetic dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
Studies on the epigenetic regulation of gene expression during germ cell development are still at the beginning stage. In the present study, we used our highly specific 5hmC-labeling and enrichment technique coupled with DNA deep-sequencing to profile the global 5hmC distribution in 8 serial stages of male germ cells during spermatogenesis, as well as in the the Sertoli cells (SE) which are the only somatic cell type inside seminiferous tubules. We analyzed the genomic distribution and dynamic changes of 5hmC during spermatogenesis. Moreover, to dissect the functional significance of 5hmC modifications for transcriptional regulation of gene expression, we also performed RNA-Seq transcriptome analysis in all of the 8 corresponding stages of male germ cells and found that 5hmC is positively correlated with gene expression.
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| Overall design |
RNA-seq: Examination of the transcriptomes during mouse spermatogenesis.
5hmC-seq: Identification of 5hmC enriched genmoic regions in mouse germ cell.
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| Contributor(s) |
Lu W, Tang F, Han C, Liao S, Gan H |
| Citation(s) |
23759713 |
| Submission date |
Jan 11, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Chunsheng Han |
| E-mail(s) |
hancs@ioz.ac.cn
|
| Phone |
86-10-64807105
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Institute of Zoology
|
| Lab |
State Key Laboratory of Reproductive Biology
|
| Street address |
1-5 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (18)
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| Relations |
| SRA |
SRP010262 |
| BioProject |
PRJNA151173 |
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