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Series GSE3497 Query DataSets for GSE3497
Status Public on Oct 22, 2005
Title Cell division cycle in a human cancer cell line (HeLa)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This experiment set contains the complete set of raw data for Whitfield et al. (2002)Mol.Biol.Cell. HeLa S3 cells were synchronized by three different methods (double thymidine block, thymidine-nocodazole block or mitotic shake-off)in five independent time courses. mRNA from the first and second double thymidine block experiments were converted to cDNA by standard methods using an oligo-dT/random hexamer mix and hybridized to 24K arrays; mRNA from the third double thymidine and thymidine-nocodazole block experiments were converted to cDNA using olido-dT alone and hybridized to 43K arrays; total RNA from the mitotic shake-off experiment was first amplified using a modified eberwine protocol, aRNA converted to cDNA by reverse transcription with random hexamer and hybridized to 43K arrays.
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc.
Keywords: Logical Set
Overall design Computed
Web link
Contributor(s) Whitfield M, Demeter J
Citation(s) 12058064
Submission date Oct 21, 2005
Last update date Mar 16, 2012
Organization Stanford Microarray Database (SMD)
Phone 650-498-6012
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (6)
Samples (114)
GSM79620 "26h Thy-Noc, Exp4"
GSM79621 "28h Thy-Noc, Exp4"
GSM79622 "30h Thy-Noc, Exp4"
BioProject PRJNA93325

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary data files not provided

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