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Series GSE3491 Query DataSets for GSE3491
Status Public on Oct 22, 2005
Title Interindividual Variability in LPS Responses
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We used an unbiased approach to identify differences in gene expression that may account for the high degree of interindividual variability in inflammatory responses to LPS in the normal human population. We measured LPS-induced cytokine production ex vivo in whole blood from 102 healthy human subjects and identified individuals who consistently showed either very high or very low responses to LPS. Comparison of gene expression profiles between the lpshigh and lpslow individuals revealed 80 genes that were differentially expressed in the presence of LPS and 21 genes that were differentially expressed in the absence of LPS (p < 0.005, ANOVA). Expression of a subset of these genes was confirmed using real-time RT-PCR. These data illustrate a novel approach to the identification of factors that determine interindividual variability in innate immune inflammatory responses.
Keywords: comparative response
Overall design Six whole blood samples (three high and three low responders to LPS) were selected from a cohort of 102 healthy individuals, exposed to media or LPS for 4 hours. RNA was isolated and hybridized with an Affymetrix array.
Contributor(s) Wurfel MM, Park WY, Radella II F, Ruzinski J, Sandstrom A, Strout J, Bumgarner RE, Martin TR
Citation(s) 16081831
Submission date Oct 20, 2005
Last update date Dec 13, 2018
Contact name Mark M Wurfel
Organization name University of Washington
Department Pulmonary & Critical Care Medicine
Lab Pulmonary Research
Street address 300 9th Ave
City Seattle
State/province WA
ZIP/Postal code 98104
Country USA
Platforms (1)
GPL8300 [HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array
Samples (12)
BioProject PRJNA93313

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