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Series GSE34716 Query DataSets for GSE34716
Status Public on Jan 03, 2013
Title Small Marine Neurotoxin Elicits Common Antibody Response in Zebrafish and Sea Lions
Organism Danio rerio
Experiment type Expression profiling by array
Summary The neurotoxic amino acid, domoic acid, is naturally produced by marine phytoplankton and presents a significant health threat to marine mammal and human populations. Currently, diagnostic tools to assess exposure are not available, yet concerns regarding health impacts associated with low-level repetitive exposure are growing. Here we applied a laboratory zebrafish model to assess exposure to asymptomatic doses of domoic acid in a nine-month low-level repetitive exposure study. Blood analyses, whole brain gene expression, and functional lymphocyte proliferation assays analyzed at 11 time points revealed a quantifiable antibody response that was temporally correlated with upregulated immune response genes and significantly increased spontaneous lymphocyte proliferation. The antibody response was further validated in field exposed California sea lions and provides the first biomarker for chronic exposure assessment.
 
Overall design Time series domoic acid exposure of zebrafish.
 
Contributor(s) Beyer RP, Bammler TK, Lefebvre KA, Frame ER, Hiolski E, Marcinek DJ
Citation(s) 22567140, 25033243
Submission date Dec 24, 2011
Last update date Aug 16, 2019
Contact name James William MacDonald
E-mail(s) jmacdon@uw.edu
Organization name University of Washington
Department Environmental and Occupational Health Sciences
Street address 4225 Roosevelt Way NE
City Seattle
State/province WA
ZIP/Postal code 98105-6099
Country USA
 
Platforms (1)
GPL14688 Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Feature Number version)
Samples (72)
GSM853615 Domoic Acid 24hrs set 1
GSM853616 Control 24hrs set 1
GSM853617 Domoic Acid 6hrs set 1
Relations
BioProject PRJNA150439

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34716_RAW.tar 652.6 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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