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Series GSE34637 Query DataSets for GSE34637
Status Public on Dec 22, 2011
Title Methylation Changes During Soybean Seed Development
Organism Glycine max
Experiment type Methylation profiling by high throughput sequencing
Summary What methylation changes are occurring during seed development largely remains unknown. To uncover the possible role of DNA methylation throughout all of seed development - from fertilization through dormancy and post-germination in soybean, we characterized the methylome of whole seeds representing the differentiation (GLOB and COT stages), maturation (early- [EM], mid- [B1] and late- [AA1] maturation stages), dormancy (DRY stage), and post-germination (seedling) phases of soybean seed development using Illumina sequencing. In addition, we characterized the methylome of the cotyledons of germinated seedling to examine methylation differences before and after germination.
 
Overall design Illumina sequencing of bisulfite-converted genomic DNA from globular stage (GLOB), cotyledon-stage (COT), early-maturation stage (EM), mid-maturation stage (B1), and late-maturation stage (AA1), pre-dormancy stage 1 (AA2), pre-dormancy stage 2 (AA3), dormancy stage (DRY) seeds, and post-germination seedling (SDLG), seedling cotyledons (SDLG-COT).
 
Contributor(s) Goldberg RB, Chen M, Lin J, Le BH
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Submission date Dec 21, 2011
Last update date Dec 07, 2016
Contact name Bob Goldberg
E-mail bobglab@mcdb.ucla.edu
Phone 310-825-3270
Organization name University of California, Los Angeles
Department Molecular, Cell and Developmental Biology
Street address 610 Charles E Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL15008 Illumina HiSeq 2000 (Glycine max)
Samples (18)
GSM852274 Globular stage whole seed
GSM852275 Early-maturation stage whole seed
GSM852278 Late-maturation stage whole seed biological replicate 1
Relations
SRA SRP010037
BioProject PRJNA151265

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34637_RAW.tar 16.6 Gb (http)(custom) TAR (of TXT)
GSE34637_processed_readme.txt 672 b (ftp)(http) TXT
Raw data are available in SRA
Processed data provided as supplementary file

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