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| Status |
Public on Jan 01, 2013 |
| Title |
Genome-wide maps of RNA Polymerase II and p65 localization in HUVECs stimulated with TNF alpha |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
TNF alpha is one of the inflammatory mediator and induce genes mainly by transcriptional factor, p65, in endothelial cells. This time, we performed a time course study to detect the change of localization of p65 and Pol II.
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| Overall design |
To identify p65 and Pol II binding sites, we used chromatin immunoprecipitation with deep sequencing (ChIP-seq) of HUVECs treated with or without TNF alpha for 30 mins. Cells were starved before stimulation longer than 16 hours. HUVECs were used within the first 6 passages. For crosslinking, 10 mM of EGS in 50% glacial acetic acid was used for 45 min, followed by 20 min of 1% paraformaldehyde treatmet was used.
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| Contributor(s) |
Youichiro W, Takahide K, Mika K, Yamamoto S, Kodama T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Dec 16, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Takahide Kohro |
| E-mail(s) |
kohro@lsbm.org
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| Phone |
81-3-5452-5237
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| Organization name |
LSBM, RCAST
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| Street address |
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| City |
4-6-1, Komaba, Meguroku |
| State/province |
Tokyo |
| ZIP/Postal code |
153-8904 |
| Country |
Japan |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (4)
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| Relations |
| SRA |
SRP009868 |
| BioProject |
PRJNA151313 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34500_RAW.tar |
105.6 Mb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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