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| Status |
Public on Dec 09, 2011 |
| Title |
Clonal Selection Drives Genetic Divergence of Metastatic Medulloblastoma [Affymetrix SNP6 Arrays] |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by SNP array
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| Summary |
Medulloblastoma, the most common malignant pediatric brain tumour, disseminates by shedding cells into the cerebrospinal fluid, which then re-implant to cover the surface of the brain and spinal cord. Metastases are a very poor prognostic sign at presentation and are usually lethal at recurrence. Mechanisms driving dissemination have been described in the bulk primary tumour, with the underlying assumption that primary tumour and metastases are biologically similar. Here we show that in both mouse and human medulloblastoma, multiple metastases from a single animal are extremely similar, but are genetically highly divergent from the primary tumour. Clonal genetic events in the metastases can be demonstrated in a restricted sub-clone of the primary tumour, suggesting that only rare cells within the primary tumour have the ability to metastasize. Failure to account for the bicompartmental nature of primary and metastatic medulloblastoma represents a major barrier to the development of effective targeted therapies.
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| Overall design |
Affymetrix SNP arrays were performed according to the manufacturer's directions on DNA extracted from cryopreserved human medulloblastoma tissue samples.
Copy number analysis of Affymetrix SNP6 arrays was performed for 17 pediatric medulloblastoma samples. Samples comprise a series of 7 patient-matched primary/metastatic cases.
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| Contributor(s) |
Northcott PA, Wu X, Taylor MD |
| Citation(s) |
22343890 |
| |
| Submission date |
Dec 08, 2011 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Paul A. Northcott |
| E-mail(s) |
panorthcott@gmail.com
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| Organization name |
German Cancer Research Center (DKFZ)
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| Street address |
Im Neuenheimer Feld 280
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| City |
HEIDELBERG |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL6801 |
[GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array |
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| Samples (17)
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| This SubSeries is part of SuperSeries: |
| GSE34356 |
Clonal Selection Drives Genetic Divergence of Metastatic Medulloblastoma |
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| Relations |
| BioProject |
PRJNA156527 |
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