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Series GSE34061 Query DataSets for GSE34061
Status Public on Jan 01, 2013
Title Progressive degeneration of human neural stem cells caused by pathogenic LRRK2
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Aging and its physiological manifestations have been correlated with adult stem cell exhaustion and a failure to maintain tissue homeostasis1-10. Since multiple morphological cellular defects are associated with aging-related disorders, we hypothesized that late onset disorders might be linked to adult stem cell abnormalities compromising cellular function over time. Our work shows that a dominant G2019S mutation in the human LRRK2 gene, which is associated with central nervous system disorders, including Parkinson’s disease, the second most prevalent neurodegenerative disease in the aging population, causes alterations in neural stem cell homeostasis in aging-related cellular contexts. They include disruption of the nuclear architecture, deficiencies in clonal expansion and alterations in neural differentiation assays as well as an increased susceptibility to proteasomal stress. These phenotypic changes are dependent on differential kinase activity manifested during cellular passaging. Our studies might open new venues for studying the influence of aging in neural stem cell dependent processes, such as cognitive impairments, in the degenerating diseased brain.
Overall design Genome-wide localization of Histon K4 trimethylation in human iPSC and iPSC-derived neural stem cell using ChIP-seq
Contributor(s) Liu G, Qu J, Suzuki K, Li M, Sancho-Martinez I, Yi F, Nivet E, Ruiz S, Montserrat N, Zhang W, Wagner U, Kim A, Ren B, Goebl A, Kim J, Soligalla RD, Dubova I, Rodriguez Esteban C, Izpisua Belmonte JC
Citation(s) 23075850
Submission date Nov 30, 2011
Last update date Mar 20, 2019
Contact name Ulrich Wagner
Organization name Uiversity Hospital Zurich
Street address Schmelzbergstrasse 12
City Zurich
ZIP/Postal code 8091
Country Switzerland
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (6)
GSM841171 LK2(+/+)-iPSC
GSM841172 LK2(GS/GS)-iPSC
GSM841173 LK2(+/+)-ipsNSC(p10)
SRA SRP009492
BioProject PRJNA150019

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Supplementary file Size Download File type/resource
GSE34061_RAW.tar 1.9 Gb (http)(custom) TAR (of BED, BW)
Raw data are available in SRA
Processed data provided as supplementary file

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