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| Status |
Public on Dec 02, 2011 |
| Title |
Reactivity of commercial antibodies targeting human histone H2B N-terminal tail on the Intel array platform |
| Platform organism |
Homo sapiens |
| Sample organism |
Oryctolagus cuniculus |
| Experiment type |
Other
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| Summary |
The aim of the study was to test the Intel array platform and map the H2B epitopes bound by commercial antibody reagents. We also tested the ability of SET7 PKMT to methylate Intel array peptides.
We diluted antibody reagents 1:1000 and incubated dilutions on the silicon-based Intel array platorm.
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| Overall design |
We mapped antibody binding epitopes and SET7 PKMT methylation target peptides using a novel, silicon-based array platform with 8820 individual peptide features comprising every possible contiguous peptide from 1-21 a.a. in length within the 21-mer N-terminal tail of human histone H2B.
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| Contributor(s) |
Price JV, Tangsombatvisit S, Xu G, Levy D, Baechler EC, Gozani O, Varma M, Utz PJ, Liu CL |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 30, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Jordan Price |
| Organization name |
Stanford University
|
| Street address |
CCSR 2250
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA148077 |
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