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| Status |
Public on Jun 18, 2012 |
| Title |
Disrupted transcriptional network in ΔNp63 AEC tissue model [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The transcriptional basis for disrupted epidermal differentiation arising from TP63 AEC mutations remains to be elucidated. Here we present an organotypic model of AEC dysfunction that phenocopies differentiation defects observed in AEC patient skin. Transcriptional analysis of model AEC tissue revealed impaired induction of differentiation regulators, including OVOL1, GRHL3, KLF4, PRDM1 and ZNF750. Genome wide binding analyses of TP63 during epidermal differentiation showed direct binding of OVOL1, GRHL3, and ZNF750 promoters suggesting AEC mutants prevent normal activation of these targets by direct transcriptional interference. Remarkably, exogenous ZNF750 restores impaired epidermal differentiation caused by AEC mutation. Thus, repression of ZNF750 is central to disrupted epidermal differentiation in model AEC tissue.
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| Overall design |
ChIP-Seq analysis: Examination of p63 binding in proliferating and differentiating human keratinocytes
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| Contributor(s) |
Zarnegar BJ, Webster DE, Hunt BV, Qu K, Yan KJ, Berk DR, Sen GL, Khavari PA |
| Citation(s) |
22922031 |
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| Submission date |
Nov 09, 2011 |
| Last update date |
Oct 11, 2022 |
| Contact name |
Douglas Porter |
| Organization name |
Stanford
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| Department |
Dermatology
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| Lab |
Khavari
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| Street address |
269 Campus Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE33572 |
Disrupted transcripitonal network in ΔNp63 AEC tissue model |
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| Relations |
| SRA |
SRP009255 |
| BioProject |
PRJNA154341 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33571_RAW.tar |
2.7 Gb |
(http)(custom) |
TAR (of BED, BW, SAM) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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