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| Status |
Public on Jun 01, 2012 |
| Title |
Peripheral Blood Monocyte Gene Expression in Recent-Onset Type 1 Diabetes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
OBJECTIVE: Novel biomarkers of disease progression after type 1 diabetes onset are needed. RESEARCH DESIGN AND METHODS: We profiled peripheral blood (PB) monocyte gene expression in 6 healthy subjects and 16 children with type 1 diabetes diagnosed ~3 months previously, and analyzed clinical features from diagnosis to 1 year. RESULTS: Monocyte expression profiles clustered into two distinct subgroups, representing mild and severe deviation from healthy controls, along the same continuum. Patients with strongly divergent monocyte gene expression had significantly higher insulin dose-adjusted HbA1c levels during the first year, compared to patients with mild deviation. The diabetes-associated expression signature identified multiple perturbations in pathways controlling cellular metabolism and survival, including endoplasmic reticulum and oxidative stress (e.g. induction of HIF1A, DDIT3, DDIT4 and GRP78). qPCR quantitation of a 9-gene panel correlated with glycaemic control in 12 additional recent-onset patients. The qPCR signature was also detected in PB from healthy first-degree relatives. CONCLUSIONS: A PB gene expression signature correlates with glycaemic control in the first year after diabetes diagnosis, and is present in at-risk subjects. These findings implicate monocyte phenotype as a candidate biomarker for disease progression pre- and post-onset, and systemic stresses as contributors to innate immune function in type 1 diabetes.
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| Overall design |
CD14+ monocytes from a total of 16 children with recent-onset type 1 diabetes and 6 adult healthy controls were profiled in 2 independent microarrays.
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| Contributor(s) |
Irvine KM |
| Citation(s) |
22403299 |
| Submission date |
Nov 03, 2011 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Katharine Irvine |
| E-mail(s) |
katharine.irvine@uq.edu.au
|
| Organization name |
Mater Research Institute UQ
|
| Street address |
Translational Research Institute
|
| City |
Brisbane |
| ZIP/Postal code |
4102 |
| Country |
Australia |
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| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA148693 |
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