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Series GSE33423 Query DataSets for GSE33423
Status Public on Mar 01, 2012
Title Environmental Toxicants Induce Epigenetic Transgenerational Inheritance of Ovarian Disease [Affymetrix]
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The epigenetic transgenerational actions of environmental toxicants and relevant mixtures on ovarian disease was investigated with the use of a fungicide, a pesticide mixture, a plastic mixture, dioxin and a hydrocarbon mixture. After transient exposure of an F0 gestating female rat during embryonic gonadal sex determination, the F1, F2 and F3 generation progeny adult onset ovarian disease was assessed. Transgenerational disease phenotypes observed included an increase in cysts resembling human polycystic ovarian disease (PCO) and a decrease in the ovarian primordial follicle pool size resembling premature ovarian failure (POF).   The F3 generation granulosa cells were isolated and found to have a transgenerational effect on the transcriptome and epigenome (differential DNA methylation). Epigenetic biomarkers for environmental exposure and associated gene networks were identified. Epigenetic transgenerational inheritance of ovarian disease states were induced by different classes of environmental compounds suggesting a role of environmental epigenetics in ovarian disease etiology.
We used transcriptome microarray analysis to determine genes expressed differentially between F3 control and F3 vinclozolin lineage rat ovary granulosa cell and see which genes might be connected to or cause observed ovary diseases
Overall design RNA samples from granulosa cell of 3 F3-control lineage groups are compared to granulosa cell of 3 F3-vinclozolin lineage groups
Contributor(s) Nilsson EE, Larsen G, Manikkam M, Guerrero-Bosagna C, Savenkova MI, Skinner MK
Citation(s) 22570695
Submission date Nov 02, 2011
Last update date Jul 10, 2014
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL6247 [RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version]
Samples (6)
GSM826747 GC_F3-Control_biological rep1
GSM826748 GC_F3-Control_biological rep2
GSM826749 GC_F3-Control_biological rep3
This SubSeries is part of SuperSeries:
GSE59278 Environmentally induced epigenetic transgenerational inheritance of ovarian disease
BioProject PRJNA148923

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Supplementary file Size Download File type/resource
GSE33423_RAW.tar 23.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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