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Series GSE33150 Query DataSets for GSE33150
Status Public on Oct 25, 2011
Title Substrate selectivity for semisynthetic CK2 proteins with Pin1
Platform organism Homo sapiens
Sample organism synthetic construct
Experiment type Protein profiling by protein array
Summary Protein Ser/Thr kinase CK2 is involved in a myriad of cellular processes including cell growth and proliferation by phosphorylating hundreds of substrates, yet the regulation process of CK2 function is poorly understood. The CK2 catalytic subunit, CK2α, is phosphorylated at Thr344 and phosphorylation on the C-terminal tail of CK2α is required for interaction with Pin1 protein. The substrate selectivity for protein kinase CK2α was examined by performing kinase assays on protein microarrays spotted with 17,000 human proteins. Semisynthetic CK2α proteins were prepared to contain an unmodified C-terminal tail or phospho-Thr (pThr) at T344. These semisynthetic proteins were used to determine if the phosphorylation-dependent interaction of CK2α with Pin1 can modulate the substrate selectivity for CK2.
The different semisynthetic CK2α proteins (unmodified and pThr344) were tested alone and in the presence of the recombinant Pin1 protein. Pin1 has been shown to interaction with CK2α only when CK2α is phoshorylated on its C-terminal site (including Thr344).
 
Overall design In the study presented here, kinase assays were performed using two different semisynthetic CK2α proteins: unmodified C-terminal tail and phospho-Thr (pThr) at 344. The semisynthetic proteins were each tested alone and in the presence of the recombinant Pin1 protein. There were four different kinase conditions and each condition was performed in duplicate.
 
Contributor(s) Tarrant MK, Cole PA, Rho H, Zhu H, Xie Z, Qian J
Citation(s) 22267120
Submission date Oct 21, 2011
Last update date Mar 23, 2012
Contact name Mary Katherine Tarrant
E-mail(s) marykatherine.tarrant@jhu.edu
Phone 410-614-0322
Organization name Johns Hopkins University School of Medicine
Department Pharmacology and Molecular Sciences
Lab Philip A. Cole
Street address 725 N. Wolfe Street, 317 Hunterian Bldg.
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platforms (1)
GPL14772 JHUHIT Human 17K v2010
Samples (8)
GSM820932 unmodified CK2α with Pin1_Rep1
GSM820933 unmodified CK2α with Pin1_Rep2
GSM820934 unmodified CK2α alone_Rep1
This SubSeries is part of SuperSeries:
GSE33151 Substrate selectivity for semisynthetic CK2 proteins
Relations
BioProject PRJNA156599

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33150_RAW.tar 15.6 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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