|
| Status |
Public on May 03, 2012 |
| Title |
Transcription factor AbVf19 induces hydrolytic enzyme genes during pathogenesis in Alternaria brassicicola |
| Organism |
Brassica oleracea |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Alternaria brassicicola is a successful saprophyte and necrotrophic pathogen with a broad host range. It produces secondary metabolites that marginally affect virulence, in contrast to many A. alternata strains that produce secondary metabolites as host-specific pathogenicity factors. Cell wall-degrading enzymes (CWDEs) have been considered important for pathogenesis, but no CWDEs have been identified as significant virulence factors in A. brassicicola. In this study, we discovered mutants of a gene, AbVf19, which consistently produced smaller lesions than the wild type. The mutants grew slower than the wild type on an axenic medium with pectin as a major carbon source. Gene expression comparisons identified several hydrolytic enzyme-coding genes being down-regulated in the mutant during a late stage of infection. These down-regulated genes comprised a small fraction of genes within each family. Three of these genes had mutants that showed no or little change in virulence. This suggested that each down regulated gene only made a small contribution to virulence, or that their functions were redundant. This study demonstrated the existence and importance of a transcription factor that regulates a suite of genes that are probably important for decomposing and utilizing plant material during the late stage of plant infection.
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| |
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| Overall design |
gene expression profile comparisons between wild type and a transcription factor mutant during host infection
|
| |
|
| Contributor(s) |
Cho Y, Ohm R |
| Citation(s) |
22185468 |
| Submission date |
Oct 21, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Yangrae Cho |
| E-mail(s) |
yangrae@hawaii.edu
|
| Phone |
808-956-5305
|
| Organization name |
University of Hawaii at Manoa
|
| Department |
Plant and Environmental Protection Sciences
|
| Street address |
3190 Maile Way, St John 317
|
| City |
Honolulu |
| State/province |
HI |
| ZIP/Postal code |
96822 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16360 |
Illumina HiSeq 2000 (Brassica oleracea) |
|
| Samples (6)
|
|
| Relations |
| SRA |
SRP009064 |
| BioProject |
PRJNA149375 |
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