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Series GSE33134 Query DataSets for GSE33134
Status Public on Dec 31, 2011
Title Protective gene expression changes elicited by an inherited defect in photoreceptor structure.
Organism Mus musculus
Experiment type Expression profiling by array
Summary To investigate pathogenic mechanisms in such instances, we have characterized rod photoreceptor and retinal gene expression changes in response to a defined insult to photoreceptor structure, using the retinal degeneration slow (rds) mouse model. Global gene expression profiling was performed on flow-sorted rds and wild-type rod photoreceptors immediately prior and subsequent to times at which OSs are normally elaborated. Dysregulated genes were identified via microarray hybridization, and selected candidates were validated using quantitative PCR analyses. We identified a single key gene, Egr1, that was dysregulated in a sustained fashion in rds rod photoreceptors and in the retina. Egr1 upregulation was associated with microglial activation and migration, into the outer retina at times subsequent to the major peak of photoreceptor cell death. Interestingly, this response was accompanied by neurotrophic factor upregulation. We hypothesize that activation of Egr1 and neurotrophic factors represents a protective immune mechanism, contributing to the characteristically slow retinal degeneration of the rds mouse model.
 
Overall design We had two conditions WT and Rds-KO at 4 different time points: postnatal (P) 6, P9, P14 and P21.
 
Contributor(s) Sharma YV, Cojocaru R, Ritter LM, Khattree N, Brooks M, Scott A, Swaroop A, Goldberg AF
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Submission date Oct 21, 2011
Last update date Feb 11, 2019
Contact name Radu Cojocaru
E-mail(s) raducojo@gmail.com
Organization name Elsevier
Street address 5635 Fishers Lane, Suite 510
City Rockville
State/province MD
ZIP/Postal code 20852
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (31)
GSM820586 Rds at post natal day 6, biological replicate 1
GSM820587 Rds at post natal day 6, biological replicate 2
GSM820588 Rds at post natal day 6, biological replicate 3
Relations
BioProject PRJNA149405

Download family Format
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Supplementary file Size Download File type/resource
GSE33134_RAW.tar 97.7 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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