 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 31, 2011 |
| Title |
Protective gene expression changes elicited by an inherited defect in photoreceptor structure. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To investigate pathogenic mechanisms in such instances, we have characterized rod photoreceptor and retinal gene expression changes in response to a defined insult to photoreceptor structure, using the retinal degeneration slow (rds) mouse model. Global gene expression profiling was performed on flow-sorted rds and wild-type rod photoreceptors immediately prior and subsequent to times at which OSs are normally elaborated. Dysregulated genes were identified via microarray hybridization, and selected candidates were validated using quantitative PCR analyses. We identified a single key gene, Egr1, that was dysregulated in a sustained fashion in rds rod photoreceptors and in the retina. Egr1 upregulation was associated with microglial activation and migration, into the outer retina at times subsequent to the major peak of photoreceptor cell death. Interestingly, this response was accompanied by neurotrophic factor upregulation. We hypothesize that activation of Egr1 and neurotrophic factors represents a protective immune mechanism, contributing to the characteristically slow retinal degeneration of the rds mouse model.
|
| |
|
| Overall design |
We had two conditions WT and Rds-KO at 4 different time points: postnatal (P) 6, P9, P14 and P21.
|
| |
|
| Contributor(s) |
Sharma YV, Cojocaru R, Ritter LM, Khattree N, Brooks M, Scott A, Swaroop A, Goldberg AF |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 21, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Radu Cojocaru |
| E-mail(s) |
raducojo@gmail.com
|
| Organization name |
Elsevier
|
| Street address |
5635 Fishers Lane, Suite 510
|
| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20852 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (31)
|
| GSM820586 |
Rds at post natal day 6, biological replicate 1 |
| GSM820587 |
Rds at post natal day 6, biological replicate 2 |
| GSM820588 |
Rds at post natal day 6, biological replicate 3 |
| GSM820589 |
Rds at post natal day 6, biological replicate 4 |
| GSM820590 |
Rds at post natal day 9, biological replicate 1 |
| GSM820591 |
Rds at post natal day 9, biological replicate 2 |
| GSM820592 |
Rds at post natal day 9, biological replicate 3 |
| GSM820593 |
Rds at post natal day 9, biological replicate 4 |
| GSM820594 |
Rds at post natal day 14, biological replicate 1 |
| GSM820595 |
Rds at post natal day 14, biological replicate 2 |
| GSM820596 |
Rds at post natal day 14, biological replicate 3 |
| GSM820597 |
Rds at post natal day 14, biological replicate 4 |
| GSM820598 |
Rds at post natal day 21, biological replicate 1 |
| GSM820599 |
Rds at post natal day 21, biological replicate 2 |
| GSM820600 |
Rds at post natal day 21, biological replicate 3 |
| GSM820601 |
Rds at post natal day 21, biological replicate 4 |
| GSM820602 |
WT at post natal day 6, biological replicate 1 |
| GSM820603 |
WT at post natal day 6, biological replicate 2 |
| GSM820604 |
WT at post natal day 6, biological replicate 3 |
| GSM820605 |
WT at post natal day 6, biological replicate 4 |
| GSM820606 |
WT at post natal day 9, biological replicate 1 |
| GSM820607 |
WT at post natal day 9, biological replicate 2 |
| GSM820608 |
WT at post natal day 9, biological replicate 3 |
| GSM820609 |
WT at post natal day 9, biological replicate 4 |
| GSM820610 |
WT at post natal day 14, biological replicate 1 |
| GSM820611 |
WT at post natal day 14, biological replicate 2 |
| GSM820612 |
WT at post natal day 14, biological replicate 3 |
| GSM820613 |
WT at post natal day 21, biological replicate 1 |
| GSM820614 |
WT at post natal day 21, biological replicate 2 |
| GSM820615 |
WT at post natal day 21, biological replicate 3 |
| GSM820616 |
WT at post natal day 21, biological replicate 4 |
|
| Relations |
| BioProject |
PRJNA149405 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE33134_RAW.tar |
97.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Raw data provided as supplementary file |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...