GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE33108 Query DataSets for GSE33108
Status Public on Oct 21, 2011
Title Role of estrogen related receptor alpha (ERRa) in CD4+ T cell gene expression
Organism Mus musculus
Experiment type Expression profiling by array
Summary ERRa is an orphan nuclear receptor with an established role in cell metabolism. Our studies demonstrate that acute or chronic loss of ERRa broadly affects mitochondrial and glycolytic metabolism in CD4+ T cells and results in diminished T cell function and differentation.
In this array, we assessed the contribution of ERRa to changes in gene expression in resting and stimulated CD4+ T cells with the goal of identifying specific metabolic genes that exhibit altered expression in the presence or absence of ERRa. Please see related publication: Michalek, et al. PNAS 2011.
Overall design CD4+ T cells from 6-8 week old mice were negatively selected (Miltenyi), resulting in >95% purity and cultured in RPMI 1640 (Mediatech) with 10% FBS (Gemini Bio-Products). Wild-type (WT) and ERRa−/− (ERRKO) CD4+ T cells were harvested directly ex vivo or stimulated with anti-CD3 and anti-CD28 antibodies for 12 h in the presence or absence of the pharmacological inhibtors of ERRa XCT790 and Compound A. RNA was harvested using the RNeasy Plus kit from Qiagen.The microarray was prepared and analyzed by the Duke Microarray Facility using Ambion Message Amp Premier labeling and Affymetrix GeneChip Mouse Genome 430A 2.0. Each assay contains three independent biologic replicates.
Contributor(s) Michalek R, Rathmell J, Kazmin D
Citation(s) 22042850
Submission date Oct 20, 2011
Last update date May 04, 2018
Contact name Dmitri A Kazmin
Phone 404-712-2594
Organization name Emory University
Department Emory Vaccine Center
Lab Pulendran
Street address 954 Gatewood Dr
City Atlanta
State/province GA
ZIP/Postal code 30329
Country USA
Platforms (1)
GPL8321 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array
Samples (18)
GSM819927 ERR WT, resting, rep.1
GSM819928 ERR WT, resting, rep. 2
GSM819929 ERR WT, resting, rep. 3
BioProject PRJNA149499

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33108_RAW.tar 35.0 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap