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Series GSE32966 Query DataSets for GSE32966
Status Public on Oct 13, 2011
Title Dermal reprogramming through epidermal activation of beta-catenin
Organism Mus musculus
Experiment type Expression profiling by array
Summary Hair follicle formation depends on reciprocal epidermal-dermal interactions and occurs during skin development, but not in adult life. This suggests that the properties of dermal fibroblasts change during postnatal development. To examine this, we used a PdgfraEGFP mouse line to isolate GFP-positive fibroblasts from neonatal skin, adult telogen and anagen skin and adult skin in which ectopic hair follicles had been induced (EF skin) by transgenic epidermal activation of beta-catenin. We also isolated epidermal cells from each mouse. The gene expression profile of EF epidermis was most similar to that of anagen epidermis, consistent with activation of beta-catenin signalling. In contrast, adult dermis with ectopic hair follicles more closely resembled neonatal dermis than adult telogen or anagen dermis. In particular, genes associated with mitosis were upregulated and extracellular matrix-associated genes were downregulated in neonatal and EF fibroblasts. We confirmed that sustained epidermal beta-catenin activation stimulated fibroblasts to proliferate to reach the high cell density of neonatal skin. In addition, the extracellular matrix was comprehensively remodelled, with mature collagen being replaced by collagen subtypes normally present only in developing skin. The changes in proliferation and extracellular matrix composition originated from a specific subpopulation of fibroblasts located beneath the sebaceous gland. Our results show that adult dermis is an unexpectedly plastic tissue that can be reprogrammed to acquire the molecular, cellular and structural characteristics of neonatal dermis in response to cues from the overlying epidermis.
 
Overall design We have isolated the following populations of cells from mouse back skin by flow cytometry: 1A) GFP+ WT neonatal dermal fibroblasts, 1B) ItgA6+ WT neonatal epidermal keratinocytes, 2A) GFP+ WT telogen dermal fibroblasts, 2B) ItgA6+ WT telogen epidermal keratinocytes, 3A) GFP+ D2 transient activation (anagen) dermal fibroblasts, 3B) ItgA6+ D2 transient activation (anagen) epidermal keratinocytes, 4A) GFP+ D2 sustained activation (ectopic follicles) dermal fibroblasts, 4B) ItgA6+ D2 sustained activation (ectopic follicles) epidermal keratinocytes
 
Contributor(s) Collins CA, Kretzschmar K, Watt FM
Citation(s) 22031549, 26121213
Submission date Oct 13, 2011
Last update date Jun 07, 2019
Contact name Kai Kretzschmar
E-mail(s) kk405@cam.ac.uk
Organization name University of Cambridge
Department Wellcome Trust Centre for Stem Cell Research
Lab Watt Lab
Street address Tennis Court Road
City Cambridge
State/province Cambridgeshire
ZIP/Postal code CB2 1QR
Country United Kingdom
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (24)
GSM816522 WT_dermis_neonatal_biological_replicate_1
GSM816523 WT_dermis_neonatal_biological_replicate_2
GSM816524 WT_dermis_neonatal_biological_replicate_3
Relations
BioProject PRJNA146527

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32966_RAW.tar 87.1 Mb (http)(custom) TAR (of CEL)
GSE32966_siggenes_dermalfibroblasts.txt.gz 211.7 Kb (ftp)(http) TXT
GSE32966_siggenes_epidermalkeratinocytes.txt.gz 106.6 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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