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Series GSE32939 Query DataSets for GSE32939
Status Public on Oct 13, 2011
Title CD4 on human monocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We examined the efffects of ligating CD4 expressed by primary human peripheral blood monocytes with soluble MHC Class II.
 
Overall design 3 replicates are included. Fresh blood monocytes, obtained through negative selection via magnetic activted cell sorting (Miltenyi, Auburn, CA), from three separate individual donors were treated with RPMI-1640 medium alone or with medium containing sMHC II (1 μg/ml) for three hours. Cells were harvested and RNA purified using the RNeasy procedure (Qiagen). RNA was then analyzed by the UCLA Clinical Microarray Core Laboratory using the Human U133 Plus 2.0 Array (Affimetrix). Data was analyzed using Dchip software. Visual inspection did not find any potential outliers or suspect areas. Data across all groups were normalized using the PM/MM difference.
 
Contributor(s) Kitchen SG
Citation(s) 24942581
Submission date Oct 12, 2011
Last update date Mar 25, 2019
Contact name Scott G. Kitchen
E-mail(s) skitchen@ucla.edu
Phone 310-983-1251
Fax 310-983-1067
Organization name UCLA AIDS Institute
Street address 615 Charles E.Young Dr. S.
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM815833 TLR6 MEDIUM ONLY
GSM815834 TLR6 SMHC II
GSM815835 TLR7 MEDIUM ONLY
Relations
BioProject PRJNA146575

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32939_RAW.tar 28.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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