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Series GSE32933 Query DataSets for GSE32933
Status Public on Jul 15, 2012
Title Exon arrays from HeLa cells transfected with RBM4-specific siRNA
Organism Homo sapiens
Experiment type Expression profiling by array
Summary RBM4 is a RNA-binding protein (RBP) able to modulate splicing by promoting exon inclusion and shares an import pathway with other splicing factors in the nucleus. In earlier studies we found that RBM4 interacts and colocalizes with WT1, which has been implicated in 10–15% of Wilms’ tumours and more recently in leukemya, is considered to be a tumour suppressor. RBM4, a RBP whose splicing effect is inhibited by the +KTS isoform of the tumour-suppressor/activator WT1, exhibits altered expression in different tumours, and may be essential for proliferation. Moreover, RBM4 binds to a number of RNAs of genes involved in acute myeloid leukaemia and cell cycle control. These results suggest that RBM4 may be involved in alternative splicing, tumorigenesis and particularly leukaemogenesis. To determine the endogenous transcripts that are targeted by RBM4 at the genome-wide level, we decided to perform exon microarray studies.
Overall design RBM4-specific siRNA has been used for knockdown of RBM4 in HeLa cells. RNA was extracted using a Qiagen RNA extraction kit from untransfected, mock and siRNA-transfected HeLa cells and quality of RNA for microarray analysis was checked using a Bioanalyzer 2100 (Agilent Technologies). Experiments were run in triplicates.
Contributor(s) Markus MA, Marques FZ, Yang YH, Morris BJ
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Submission date Oct 12, 2011
Last update date Jul 10, 2014
Contact name Prof.Brian J Morris
Organization name University of Sydney
Department Physiology
Lab Basic & Clinical Genomics Lab
Street address Anderson Stuart Building, room N452, University of Sydney
City Sydney
State/province NSW
ZIP/Postal code 2006
Country Australia
Platforms (1)
GPL5188 [HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version]
Samples (12)
GSM815585 Untransfected cells, biological rep1
GSM815586 Untransfected cells, biological rep2
GSM815587 Untransfected cells, biological rep3
BioProject PRJNA146563

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Supplementary file Size Download File type/resource
GSE32933_RAW.tar 265.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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