|
| Status |
Public on Dec 01, 2011 |
| Title |
Human bronchial epithelial cells exposed to A. alternata spores |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This present study is the first to investigate the global changes in host gene expression during the interaction of human bronchial epithelial cells and live Alternaria spores. Human bronchial epithelial cells (BEAS2-B) were exposed to spores or media alone for 24 hours. RNA was collected from three biological replicates/treatment and used to assess changes in gene expression patterns using Affymetrix Human Genome U133 Plus 2.0 Arrays. Interestingly, many cytokine/chemokine immune response genes were upregulated. Genes involved in cell death, retinoic acid signaling, TLR3, and interferon response pathways were also significantly upregulated.
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| |
|
| Overall design |
Three biological replicates for control (untreated cells) and treatment (5 x 10^5 A. alternata spores)
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| |
|
| Contributor(s) |
Babiceanu MC, Rumore AC, Howard BA, Kita H, Lawrence CB |
| Citation(s) |
23882263 |
| |
| Submission date |
Oct 11, 2011 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Christopher B Lawrence |
| E-mail(s) |
lawrence@vbi.vt.edu
|
| Organization name |
Virginia Bioinformatics Institute, Virginia Tech
|
| Street address |
Washington Street
|
| City |
Blacksburg |
| State/province |
Virginia |
| ZIP/Postal code |
24061 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (6)
|
| GSM814049 |
human bronchial epithelial cells exposed to A. alternata spores 1 |
| GSM814050 |
human bronchial epithelial cells exposed to A. alternata spores 2 |
| GSM814051 |
human bronchial epithelial cells exposed to A. alternata spores 3 |
|
| Relations |
| BioProject |
PRJNA146633 |
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