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| Status |
Public on Aug 31, 2012 |
| Title |
Oxidative burden and mitochondrial dysfunction in a mouse model of Rett syndrome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To identify the detailed molecular causes of the mitochondrial dysfunction, oxidative burden and more vulnerable redox balance in Rett mouse hippocampus, we screened for differential gene expression in the hippocampal CA1 subfield of adult male mice. A whole mouse genome microarray was performed to assess, whether key enzymes of the mitochondrial respiratory chain or major cellular radical scavenging enzymes are affected in this MeCP2-deficient mouse model of Rett syndrome.
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| Overall design |
Comparison of gene expression between male wildtype mice (Mecp2+/y) and mice lacking the MECP2 gene (Mecp2-/y) [B6.129P2(C)-Mecp2tm-1-1Bird (Guy et al. 2001, Nat. Genet. 27: 322-326)]. In total, 9 mice of each genotype were analyzed. The mRNA of groups of 3 animals was pooled to generate 3 independent biological samples, and finally three microarrays were run for each genotype.
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| Contributor(s) |
Großer E, Fischer M, Hildebrandt B, Menzfeld C, Vogelgesang S, Manzke TU, Opitz L, Salinas-Riester G, Müller M |
| Citation(s) |
22750529 |
| |
| Submission date |
Oct 11, 2011 |
| Last update date |
Jan 19, 2018 |
| Contact name |
Gabriela Salinas |
| E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
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| Organization name |
Universitaetsmedizin Goettingen
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| Department |
Department of Pathology
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| Lab |
NGS Integrative Genomics
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| Street address |
Kreuzbergring 57
|
| City |
Goettingen |
| State/province |
Lower-Saxony |
| ZIP/Postal code |
37075 |
| Country |
Germany |
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| Platforms (1) |
| GPL10333 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA146559 |
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