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Series GSE32789 Query DataSets for GSE32789
Status Public on Oct 13, 2011
Title H3K27Me3 (Abcam2).BG3.CP190_RNAi
Project modENCODE
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary modENCODE_submission_3680
This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: The goal of these experiments are to validate and confirm the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. To do this, we are using RNAi to deplete individual non-histone chromosomal proteins in Drosophila BG3 and S2 tissue culture cells, and then using antibodies to perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. Comparison of a protein factor's binding profiles before and after depletion will increase the confidence of our predictions.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
 
Overall design EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: ML-DmBG3-c2; Tissue: CNS-derived cell-line; Developmental Stage: third instar larval stage; Genotype: y v f mal; Sex: Unknown; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line ML-DmBG3-c2; Antibody H3K27Me3 (Abcam2) (target is H3K27Me3); dsRNA (RNAi_reagent) CG6384_RNAi&oldid=39664
Web link http://www.modencode.org
http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
 
Contributor(s) Karpen G, Elgin S, Gortchakov A, Shanower G, Tolstorukov M, Kharchenko P, Kuroda M, Pirrotta V, Park P, Minoda A, Riddle N, Schwartz Y, Alekseyenko A, Kennedy C
Citation(s) 21177972, 22767387
BioProject PRJNA63467
Submission date Oct 11, 2011
Last update date Jul 08, 2015
Contact name DCC modENCODE
E-mail help@modencode.org
Phone 416-673-8579
Organization name Ontario Institute for Cancer Research
Lab modENCODE DCC
Street address MaRS Centre, South Tower, 101 College Street, Suite 800
City Toronto
State/province Ontario
ZIP/Postal code M5G 0A3
Country Canada
 
Platforms (1)
GPL6629 [DM_tiling2_MR] Affymetrix Drosophila Tiling 2.0R Array
Samples (4)
GSM811992 expt.1229 extraction1_array1
GSM811993 expt.1235 extraction2_array1
GSM811994 expt.746 extraction3_array1

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32789_1229.Mvalues.bedgraph.gz 40.3 Mb (ftp)(http) BEDGRAPH
GSE32789_746.Mvalues.bedgraph.gz 41.3 Mb (ftp)(http) BEDGRAPH
GSE32789_RAW.tar 100.9 Mb (http)(custom) TAR (of CEL)
GSE32789_repset.9702642.smoothedM.bedgraph.gz 41.5 Mb (ftp)(http) BEDGRAPH
GSE32789_repset.9702642.smoothedM.clusters.gff3.gz 147.5 Kb (ftp)(http) GFF3
Raw data provided as supplementary file
Processed data is available on Series record

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