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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 14, 2026 |
| Title |
Heat shock factor 5 is indispensable for pachynema progression and LINE1 silencing during spermatogenesis in mice [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pachytene spermatocytes were isolated from adult wild-type and Hsf5 knockout mouse testes using STA-PUT velocity sedimentation. Single-cell suspensions were prepared by enzymatic digestion of seminiferous tubules with collagenase and trypsin. Cells were loaded onto a 4%–2%–0.5% BSA gradient and allowed to sediment for 1 hour and 45 minutes at 4°C. Fractions were collected, and pachytene spermatocytes were identified by SYCP3, γH2AX, and DAPI staining under microscopy. Enriched pachytene cells were pooled and processed for RNA-Seq.
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| Overall design |
This study aims to perform RNA-seq profiling to compare purified pachytene spermatocytes from wild-type mice and their knockdown counterparts, thereby exploring gene expression changes during the pachytene stage of meiosis and spermatogenesis.
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| Contributor(s) |
Sun Q, Cao Y, Chen Z, Shen J, Li Z, Wang B, Cheng M, Chen J, Gong W, Luo M, Zhang Y, Liu R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 09, 2026 |
| Last update date |
Apr 14, 2026 |
| Contact name |
shen jin |
| E-mail(s) |
luolab308@gmail.com
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| Phone |
18163515124
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| Organization name |
Wuhan university
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| Street address |
No. 299, Bayi Road, Wuchang District, Wuhan City
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430072 |
| Country |
China |
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| Platforms (1) |
| GPL34290 |
Illumina NovaSeq X Plus (Mus musculus) |
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| Samples (4)
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| GSM9661646 |
pachytene spermatocytes, Wild type, biol rep1 |
| GSM9661647 |
pachytene spermatocytes, Wild type, biol rep2 |
| GSM9661648 |
pachytene spermatocytes, Hsf5 knockdown, biol rep1 |
| GSM9661649 |
pachytene spermatocytes, Hsf5 knockdown, biol rep2 |
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| Relations |
| BioProject |
PRJNA1451226 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE327567_Hsf5_RNAseq_Gene_count.txt.gz |
408.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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