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Series GSE32529 Query DataSets for GSE32529
Status Public on Oct 05, 2011
Title Mouse ischemic tolerance genomic analysis of the brain and blood.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Ischemic tolerance can be induced by numerous preconditioning stimuli, including various Toll-like receptor (TLR) ligands. We have shown previously that systemic administration of the TLR4 ligand, lipopolysaccharide (LPS) or the TLR9 ligand, unmethylated CpG ODNs prior to transient brain ischemia in mice confers substantial protection against ischemic damage. To elucidate the molecular mechanisms of preconditioning, we compared brain and blood genomic profiles in response to preconditioning with these TLR ligands and to preconditioning via exposure to brief ischemia.
The experiment is a comparison of multiple treatment groups with sampling at multiple time points. The objective is to identify differentially regulated genes associated with preconditioning. Time points are examined both following preconditioning alone and following subsequent ischemic challenge (middle cerebral artery occlusion (MCAO)).
Brain ipsilateral cortex tissue and blood were collected and processed from each animal.
Overall design 6 experimental conditions: (n=3-4 mice/condition) LPS treated (i.p. 0.2mg/kg) + ischemic challenge (45min MCAO) CpG treated (i.p. 0.8mg/kg) + ischemic challenge (45min MCAO) Saline treated (i.p.) + ischemic challenge (45min MCAO) brief ischemia (12 min MCAO) + ischemic challenge (45min MCAO) Sham of brief ischemia (12 min) + ischemic challenge (45min MCAO) Non-treated + ischemic challenge (45min MCAO) Time points: Pre-ischemic challenge 3hr 24hr 72hr Post-ischemic challenge 3hr 24hr Unhandled (6 mice)-BASELINE
Contributor(s) Stenzel-Poore MP, Stevens SL
Citation(s) 21653850, 21999375
Submission date Sep 30, 2011
Last update date Feb 11, 2019
Contact name Susan L Stevens
Organization name Oregon Health & Science University
Department Molecular Microbiology & Immunology
Lab Mary Stenzel-Poore
Street address 3181 SW Sam Jackson Pk Rd
City Portland
State/province OR
ZIP/Postal code 97239
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (224)
GSM805644 brain-LPS-3h-1
GSM805645 brain-LPS-3h-2
GSM805646 brain-LPS-3h-3
BioProject PRJNA147821

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32529_RAW.tar 862.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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