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| Status |
Public on Dec 06, 2011 |
| Title |
Human PC-3 prostate cancer cells: Control (shNTC; non-target shRNA) vs. shETV4 knock down (shETV4) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of human PC-3 prostate cancer cells lentivirally infected with non-target control (NTC) short hairpin (sh)RNA comparing with lentivirally shRNA mediated human ETV4 knock-down. Cells were either cultured for 24 hours at 20% oxygen tension or 0.2% oxygen. Goal was to determine (i) genes affected by hypoxia in PC-3 NTC cells and (ii) identification of hypoxically induced genes requiring ETV4 for hypoxic regulation.
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| |
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| Overall design |
four condition experiment, shNTC at 20% and 0.2% oxygen and shETV4 at 20% and 0.2% oxygen for 24 hours. Biological replicates: 3 for each of the 4 conditions
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| |
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| Contributor(s) |
Wollenick K, Wenger RH, Stiehl DP |
| Citation(s) |
22075993 |
| |
| Submission date |
Sep 26, 2011 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Daniel Philipp Stiehl |
| E-mail(s) |
kristin.wollenick@uzh.ch
|
| Organization name |
University of Zurich
|
| Department |
Physiology
|
| Street address |
Winterthurerstr. 190
|
| City |
Zürich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA147965 |
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