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Series GSE32349 Query DataSets for GSE32349
Status Public on Oct 07, 2011
Title Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin (ChIP-Seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Multiple signaling pathways ultimately modulate the epigenetic information embedded in the chromatin of gene promoters by recruiting epigenetic enzymes. We found that, in estrogen-regulated gene programming, the acetyltransferase CREB-binding protein (CBP) is specifically and exclusively methylated by the coactivator-associated arginine methyltransferase (CARM1) in vivo. CARM1-dependent CBP methylation and p160 coactivators were required for estrogen-induced recruitment to chromatin targets. Notably, methylation increased the histone acetyltransferase (HAT) activity of CBP and stimulated its autoacetylation. Comparative genome-wide chromatin immunoprecipitation sequencing (ChIP-seq) studies revealed a variety of patterns by which p160, CBP, and methyl-CBP (meCBP) are recruited (or not) by estrogen to chromatin targets. Moreover, significant target gene-specific variation in the recruitment of (1) the p160 RAC3 protein, (2) the fraction of a given meCBP species within the total CBP, and (3) the relative recruitment of different meCBP species suggests the existence of a target gene-specific “fingerprint” for coregulator recruitment. Crossing ChIP-seq and transcriptomics profiles revealed the existence of meCBP “hubs” within the network of estrogen-regulated genes. Together, our data provide evidence for an unprecedented mechanism by which CARM1-dependent CBP methylation results in gene-selective association of estrogen-recruited meCBP species with different HAT activities and specifies distinct target gene hubs, thus diversifying estrogen receptor programming.
Overall design Examination of estrogen-induced binding site in H3396 cells under Estrogen (E2) or Ethanol (EtOH) treatment: CBP in E2; CBP in EtOH (two technical replicate); CBPR742m in E2 (two technical replicates); CBPR768m in E2; CBPR768m in EtOH; CBPR2151m in E2; CBPR2151m in EtOH; Estrogen receptor alpha (ERa) in E2; ERa in EtOH; Total Histone 3 and 4 acetylated in E2; H3K18ac in E2; H3K18ac in EtOH; Polymerase II (PolII) in E2; PolII in EtOH; RAC3 in E2; Input DNA sample in E2
Contributor(s) Ceschin DG, Walia M, Wenk SS, Duboé C, Gaudon C, Xiao Y, Fauquier L, Sankar M, Vandel L, Gronemeyer H
Citation(s) 21632823
Submission date Sep 23, 2011
Last update date May 15, 2019
Contact name Marco Antonio Mendoza-Parra
Organization name IGBMC
Street address 1, rue Laurent Fries
City Illkirch; Strasbourg
ZIP/Postal code 67400
Country France
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (18)
GSM801049 CBP E2
GSM801050 CBP EtOH
GSM801051 CBPR742m E2
This SubSeries is part of SuperSeries:
GSE32350 Methylation specifies distinct estrogen-induced binding site repertoires of CBP to chromatin
SRA SRP008778
BioProject PRJNA154905

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Supplementary file Size Download File type/resource
GSE32349_RAW.tar 1.4 Gb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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