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Series GSE32334 Query DataSets for GSE32334
Status Public on Feb 16, 2012
Title Developmental timecourse of mouse ocular lens and whole embryo control
Organism Mus musculus
Experiment type Expression profiling by array
Summary Identification of genes involved in ocular birth defects remains a challenge. To facilitate the identification of genes associated with cataract, we developed iSyTE (integrated Systems Tool for Eye gene discovery; iSyTE contains microarray gene expression profiles of the mouse embryonic lens as it transitions from the stage of placode invagination to that of vesicle formation. We identified differentially regulated genes by comparing lens microarray profiles to those representing whole embryonic body (WB) without ocular tissue. These were then utilized to generate a ranked list of lens-genes enrichment, which can be viewed as iSyTE tracks in the UCSC Genome browser to aid identification of genes with lens function.
Overall design We microdissected embryonic lens from mice at E10.5, E11.5, and E12.5 (triplicate at each time point). To have a proper control, we also generated gene expression profiles of whole embryonic body (WB) at these time points. For comparative analysis, we also generated gene expression profiles of E13.5 tooth germs tissues, and matched WB. These profiles are used to identify lens and tooth specific gene expression enrichment at these embryonic time points. This dataset is then used to prioritize analysis of candidate cataract associated genes.
Contributor(s) Lachke SA, Ho JW, Kryukov GV, O'Connell DJ, Maas RL
Citation(s) 22323457
Submission date Sep 23, 2011
Last update date Feb 11, 2019
Contact name Joshua WK Ho
Organization name Brigham and Women's Hospital
Department Department of Medicine
Lab Division of Genetics
Street address 41 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (19)
GSM800869 Lens_E10.5_rep1
GSM800870 Lens_E10.5_rep2
GSM800871 Lens_E10.5_rep3
BioProject PRJNA147269

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Supplementary file Size Download File type/resource
GSE32334_RAW.tar 91.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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