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Series GSE3218 Query DataSets for GSE3218
Status Public on Nov 10, 2005
Title Expression Profiling of Adult Male Germ Cell Tumors
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Expression profiling of a panel of 101 adult male germ cell tumors and 5 normal testis specimens was performed on Affymetrix U133A and U133B microarrays. This data has been used to:
1) generate a gene classifier that predicts histology (see PMID 15870693).
2) identify candidate target genes on 12p, a region that is gained in almost 100% of germ cell tumors (see PMID 16424014)
3) identify pluripotency associated genes through comparison of pluripotent embryonal carcinoma vs. undifferentiated seminoma.
4) Identification of genes associated with patient outcome and cisplatin resistance.
Keywords: germ cell tumor, histologic subtypes
Overall design Tumor tissues were collected under IRB-approved protocols at the Memorial Sloan-Kettering Cancer Center, New York, between 1987 and 1999. The tumor samples consist of 17 seminomas, 15 pure EC, 15 pure T, 10 pure YS, 2 pure CC, and 42 NSGCT with mixed histologies. There were six patients who had each had two tumors per patient that were included in this study (designated with the same number but different letter- e.g. 155A and 155C are from same patient but represent two different tumors, one being the primary testicular lesion, the other being a liver metastasis). Five normal testis specimens (and one pooled sample consisting of equal amounts of the 5 normal testis specimens) from individual of similar ages were used as controls.
Contributor(s) Korkola JE, Houldsworth J, Chadalavada R, Olshen AB, Dobrzynski D, Reuter VE, Bosl GJ, Chaganti R
Citation(s) 16424014, 25336442
Submission date Aug 29, 2005
Last update date Aug 10, 2018
Contact name James Korkola
Phone 212-639-8281
Organization name Memorial Sloan-Kettering
Department Cell Biology
Lab Chaganti
Street address 1275 York Ave
City New York
State/province NY
ZIP/Postal code 10021
Country USA
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL97 [HG-U133B] Affymetrix Human Genome U133B Array
Samples (214)
GSM72287 germ cell tumors (GCT) and normal controls 052B 1
GSM72288 germ cell tumors (GCT) and normal controls 155A 1
GSM72289 germ cell tumors (GCT) and normal controls 155C 1
BioProject PRJNA92737

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Supplementary file Size Download File type/resource
GSE3218_RAW.tar 730.1 Mb (http)(custom) TAR (of CEL, EXP)
Raw data provided as supplementary file

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