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Series GSE31796 Query DataSets for GSE31796
Status Public on Dec 04, 2012
Title Creation of an anti-inflammatory GR cistrome by TLR4 signaling
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary An unresolved molecular paradox is how the glucocorticoid receptor (GR) activates some genes while potently repressing others. We carried out genome-wide localization and expression profiling experiments in primary bone marrow-derived mouse macrophages treated with Dexamethasone in the presence or absence of LPS. Unexpectedly, we find that the anti-inflammatory GR cistrome, which is principally composed of 'canonical' GREs colocalizing with NFkB and AP-1 co-enriched with the myeloid lineage factors C/EBP and Pu.1, is shaped by TLR4-directed chromatin dynamics, suggesting that context rather than sequence may be a critical determinant of function.
Overall design Identification of GR, cJun, NFkB(p65) binding sites in primary bone-marrow derived macrophages unstimulated and LPS-stimulated (3hrs) that were untreated or pre-treated with Dexamethasone for 16 hrs
Contributor(s) Yu RT, Uhlenhaut NH, Evans RM
Citation(s) 23159735
Submission date Aug 31, 2011
Last update date May 15, 2019
Contact name Ruth T Yu
Organization name Salk Institute
Department Gene Expression Lab
Lab Ronald Evans
Street address 10010 N Torrey Pines Rd
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (9)
GSM788650 GR-ChIP + 1uM Dex
GSM788651 GR-ChIP + 1uM Dex + LPS
GSM788652 NF-kB/p65-ChIP + 1uM Dex +LPS
SRA SRP008124
BioProject PRJNA145469

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Supplementary file Size Download File type/resource
GSE31796_RAW.tar 7.2 Gb (http)(custom) TAR (of BEDGRAPH, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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