 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 08, 2011 |
| Title |
Transcriptome-Wide Binding Sites for Components of the Saccharomyces cerevisiae Non-poly(A) termination Pathway: Nrd1, Nab3 and Sen1 |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
|
| Summary |
We report here the transcriptome-wide distribution of yeast Rpb2, Sen1, Nrd1 and Nab3 binding sites. These data sets provide highresolution definition of non-poly(A) terminators, identify novel genes regulated by attenuation of nascent transcripts close to the promoter, and demonstrate the widespread occurrence of Nrd1-bound 3'-antisense transcripts on genes that are poorly expressed. In addition, we show that Sen1 does not cross-link to many expected ncRNAs but surprisingly binds to pre-mRNA transcripts suggesting a role in 3' end formation and/or termination.
|
| |
|
| Overall design |
Six samples by adaptation of PAR-ClIP procedure
|
| |
|
| Contributor(s) |
Jamonnak N, Creamer T, Darby M, Schaughency P, Hao H, Wheelan S, Corden J |
| Citation(s) |
21954178 |
| |
| Submission date |
Aug 30, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Jeff Corden |
| E-mail(s) |
jcorden@jhmi.edu
|
| Organization name |
JHU
|
| Department |
Molecular Bio and Gen
|
| Lab |
Corden Lab
|
| Street address |
725 N Wolfe Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21211 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13272 |
Illumina Genome Analyzer IIx (Saccharomyces cerevisiae) |
|
| Samples (6)
|
| GSM791763 |
Sen1 HTB with Arc Lamp Crosslinking |
| GSM791764 |
Nrd1 HTB with Arc Lamp Crosslinking |
| GSM791765 |
Rpb2 HTB with Arc Lamp Crosslinking |
| GSM791766 |
Nrd1 HTB with Stratalinker Crosslinking |
| GSM791767 |
Nab3 HTB with Stratalinker Crosslinking |
| GSM791768 |
Nrd1 HTB Glucose Depletion with Arc Lamp Crosslinking |
|
| Relations |
| SRA |
SRP008118 |
| BioProject |
PRJNA145183 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31764_RAW.tar |
16.0 Mb |
(http)(custom) |
TAR (of WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...