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Series GSE31667 Query DataSets for GSE31667
Status Public on May 14, 2012
Title Identification of microRNAs association with Rheumatoid Arthritis Synovial Fibroblasts using the Human TNF Transgenic Mouse Model
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary OBJECTIVE: MicroRNAs (miRNAs, miRs), a class of small non-coding RNA molecules, are posttranscriptional regulators involved in a plethora of cellular functions and have been proposed as potential therapeutic targets in various diseases, including rheumatoid arthritis (RA). In this study, we sought to discover novel miR associations in synovial fibroblasts (SFs), a key cell type mediating RA pathogenesis, by performing miR expression profiling on cells isolated from the human TNF transgenic mouse model (TghuTNF or Tg197). METHODS: miR expression in SFs isolated from 8-week-old, fully diseased TghuTNF and WT littermate control mice were determined by deep sequencing of small RNAs and the arthritic profile was established by pairwise comparisons of the two groups. qRT-PCR analysis was utilised for profile validation purposes and miR quantitation in patient SFs. Dysregulated miR target genes and pathways were predicted via bioinformatic algorithms.
 
Overall design Synovial Fibroblasts isolated from TghuTNF mice (2 x biological replicates) and control WT littermate mice (2 x biological replicates)
 
Contributor(s) Pandis I, Karagianni N, Denis MC, Reczko M, Camps C, Hatzigeorgiou AG, Ragoussis J, Kollias G
Citation(s) 22562984
Submission date Aug 25, 2011
Last update date Jul 20, 2018
Contact name Martin Reczko
Organization name BSRC A. Fleming
Department Institute of Molecular Oncology
Street address 34 Fleming Street
City Varkiza
ZIP/Postal code 16602
Country Greece
 
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (4)
GSM786178 Synovial fibroblast_WT_1
GSM786179 Synovial fibroblast_WT_2
GSM786180 Synovial fibroblast_Tg_1
Relations
SRA SRP007941
BioProject PRJNA145387

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31667_RAW.tar 40.0 Kb (http)(custom) TAR (of TXT)
Raw data are available in SRA
Processed data provided as supplementary file

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