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| Status |
Public on Jan 18, 2013 |
| Title |
Centromere-Like Regions in Budding Yeast |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Accurate chromosome segregation requires centromeres (CENs), the chromosomal sites where kinetochores form, to bridge DNA and attach to microtubules. In contrast to most eukaryotes, Saccharomyces cerevisiae possesses sequence-defined point centromeres. Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) of four kinetochore components reveals regions of overlapping, extra-centromeric protein localization upon overproduction of the centromeric histone, Cse4 (CENP-A or CenH3). These identified sequences enhance proper plasmid and chromosome segregation, and are termed Centromere-like Regions (CLRs). CLRs form in close proximity to S. cerevisiae CENs and share characteristics typical of point and regional centromeres. CLR sequences are conserved among related budding yeasts, suggesting a role in vivo. These studies provide new insights into the origin and evolution of centromeres.
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| Overall design |
ChIP-Seq analysis of the kinetochore components Cse4, Mif2, Ndc10 and Ndc80 in budding yeast strains (Saccharomyces cerevisiae) with normal and elevated levels of Cse4
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| Contributor(s) |
Lefrancois P, Auerbach RK, Yellman CM, Roeder GS, Snyder M |
| Citation(s) |
23349633 |
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| Submission date |
Aug 18, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Raymond K Auerbach |
| Organization name |
Yale University
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| Street address |
266 Whitney Ave
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
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| Platforms (1) |
| GPL9377 |
Illumina Genome Analyzer II (Saccharomyces cerevisiae) |
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| Samples (13)
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| Relations |
| SRA |
SRP007869 |
| BioProject |
PRJNA145663 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31466_RAW.tar |
1.3 Gb |
(http)(custom) |
TAR (of BED, SGR, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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