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Series GSE31417 Query DataSets for GSE31417
Status Public on Jul 31, 2012
Title Genome-wide study of HCFC1 binding sites and its associated transcription factors in cycling Human HeLa cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Analysis of transcriptional regulation in human cells has implicated a large number of promoter-specific DNA-binding proteins that regulate transcription via diverse mechanisms. In some cases, these DNA-sequence-specific factors associate with intermediaries that orchestrate interactions with the chromatin-modifying enzymes. One such intermediary is HCF-1 (host-cell factor-1; HCFC1). HCF-1, first identified for its involvement in herpes-simplex virus transcription and subsequently shown to be an important cell-cycle regulator, has a poorly defined role in genome-wide transcriptional regulation. We show here, by chromatin immunoprecipitation followed by high-throughput sequence analysis (ChIP-seq), that HCF-1 is a major transcriptional start site associated factor, whose promoter association correlates positively with transcriptional activity. Thus, in HeLa cells HCF-1 is observed on 5400 generally active CpG-island promoters. Examination of the DNA sequences underlying the HCF-1-binding sites revealed three sequence motifs associated with the binding of (i) ZNF143 and Ronin/THAP11, (ii) GABP, and (iii) YY1 sequence-specific transcription factors. Subsequent ChIP-seq analysis of these four transcription factors revealed a large co-association of HCF-1 with these four transcription factors at approximately 90% of HCF-1-bound promoters. These studies suggest that a relatively small number of transcription factors -- some (ZNF143 and Ronin/THAP11) in novel combinations -- play a major role in HeLa-cell transcriptional regulation in association with HCF-1.
Overall design This experiment includes the sequencing data of material obtained from chromatin immunoprecipitation using antibodies against HCFC1(antibodies against the C-subunit and the N-subunit), PolII(antibody against the RPB2 subunit), H3K36ME3 and H3K4Me3 histone modifications, ZNF143, THAP11, GABPalpha and YY1. It also includes control data of the Input material. All of them were done using HeLa cycling cells.
Contributor(s) Michaud J, Praz V, JnBaptiste C, Herr W
Citation(s) 23539139
Submission date Aug 16, 2011
Last update date May 15, 2019
Contact name Viviane Praz
Organization name University of Lausanne
Department CIG
Lab Hernandez
Street address GĂ©nopode
City Lausanne
State/province VD
ZIP/Postal code 1015
Country Switzerland
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (12)
GSM779425 HCFC1_ChIPSeq_seqs1-6
GSM779426 HCFC1_N_ChIPSeq_seq6
GSM779427 Input_DNA_seq1
This SubSeries is part of SuperSeries:
GSE31419 The epigenetic cell-cycle regulator HCF-1 is recruited to active CpG island-containing promoters together with the ZNF143, THAP11(Ronin), YY-1 and GABP transcription factors.
SRA SRP007893
BioProject PRJNA154023

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31417_Input_cycling_aligned.bed.gz 204.5 Mb (ftp)(http) BED
GSE31417_RAW.tar 2.5 Gb (http)(custom) TAR (of BED, BEDGRAPH)
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Processed data provided as supplementary file
Raw data are available in SRA
Processed data are available on Series record

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