|
| Status |
Public on Aug 10, 2011 |
| Title |
Small RNAs in S. cerevisiae reconstituted with RNAi |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
The RNA interference (RNAi) pathway is found in most eukaryotic lineages but curiously is absent in others, including that of Saccharomyces cerevisiae. Here, we show that reconstituting RNAi in S. cerevisiae causes loss of a beneficial dsRNA virus, known as killer virus. Incompatibility between RNAi and killer viruses extends to other fungal species, in that RNAi is absent in all species known to possess dsRNA killer viruses, whereas killer viruses are absent in closely related species that retained RNAi. Thus, the advantage imparted by acquiring and retaining killer viruses explains the persistence of RNAi-deficient species during fungal evolution.
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| |
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| Overall design |
Employ high-throughput sequencing of endogenous small RNAs from Saccharomyces cerevisiae wild-type and RNAi-reconstituted strains.
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| |
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| Contributor(s) |
Drinnenberg IA, Fink GR, Bartel DP |
| Citation(s) |
21921191 |
| |
| Submission date |
Aug 09, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Ines Anna Drinnenberg |
| E-mail(s) |
drinnenb@wi.mit.edu
|
| Organization name |
Whitehead Institute for Biomedical Research
|
| Lab |
Bartel
|
| Street address |
9 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9134 |
Illumina Genome Analyzer (Saccharomyces cerevisiae) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE31300 |
Compatibility with Killer explains the Rise of RNAi-deficient Fungi |
|
| Relations |
| SRA |
SRP007877 |
| BioProject |
PRJNA154129 |
