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Series GSE31216 Query DataSets for GSE31216
Status Public on Dec 27, 2012
Title The ets transcription factor ELF5 suppresses the estrogen sensitive phenotype and contributes to antiestrogen resistance in luminal breast cancer. [human ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The ets transcription factor ELF5 specifies the differentiation of mammary progenitor cells to establish the milk-secreting lineage. ER- and poor prognosis basal breast cancers arise from this progenitor cell and these cancers express high levels of Elf5. Knockdown of ELF5 expression in basal breast cancer cell lines, or forced expression in luminal breast cancer cell lines, resulted in reduced cell proliferation. Transcript profiling and chromatin immunoprecipitation revealed that the transcriptional activity of ELF5 specified the gene expression patterns that distinguish basal from luminal breast cancer, including suppression of FOXA1, GATA3 and ER, key estrogen-action genes. Tamoxifen treatment of luminal MCF7 cells upregulated Elf5 expression and cells that acquired resistance to Tamoxifen became dependent on ELF5 for proliferation. ELF5 is a regulator of breast cancer cell proliferation, transcriptionally specifies the basal molecular subtype and is utilised by ER+ breast cancer cells to escape proliferative arrest caused by Tamoxifen.
Overall design ChIP-Seq using an antibody to ELF5, in T47D breast carcinoma cell lines
Contributor(s) Kalyuga M, Gallego-Ortega D, Lee H, Cowley MJ, Caldon L, Stone A, Allerdice S, Valdes-Mora F, Launchbury R, Statham A, Armstrong N, Alles C, Young A, Egger A, Au W, Piggin C, Evans C, Brummer T, Kaplan W, Gee JM, Nicholson RI, Sutherland RL, Swarbrick A, Naylor MJ, Clark S, Carroll J, Ormandy CJ
Citation(s) 23300383
Submission date Aug 04, 2011
Last update date May 15, 2019
Contact name Mark Cowley
Organization name Garvan Institute of Medical Research
Department Genome Informatics & Clinical Genomics
Lab Dinger lab
Street address 384 Victoria St.
City Darlinghurst
State/province NSW
ZIP/Postal code 2010
Country Australia
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (4)
GSM773912 T47D-overexpressing ELF5-ELF5 DNA-24hours-rep1
GSM773913 T47D-overexpressing ELF5-ELF5 DNA-48hours-rep1
GSM1155147 T47D-overexpressing ELF5-input DNA-24hour
This SubSeries is part of SuperSeries:
GSE30407 The ets transcription factor ELF5 suppresses the estrogen sensitive phenotype and contributes to antiestrogen resistance in luminal breast cancer.
SRA SRP007958
BioProject PRJNA154119

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Supplementary file Size Download File type/resource
GSE31216_RAW.tar 1.7 Gb (http)(custom) TAR (of BED, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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