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Series GSE31090 Query DataSets for GSE31090
Status Public on Oct 17, 2011
Title Integrated transcriptomic and proteomic profiling of white spruce stems during the transition from active growth to dormancy.
Organism Picea glauca
Experiment type Expression profiling by array
Summary In the autumn, stems of woody perennials such as forest trees undergo a transition from active growth to dormancy. We used microarray transcriptomic profiling in combination with a proteomics analysis to elucidate processes that occur during this growth-to-dormancy transition in a conifer, white spruce (Picea glauca [Moench] Voss). Several differentially expressed genes were likely associated with the developmental transition that occurs during growth cessation in the cambial zone and the concomitant completion of cell maturation in vascular tissues. Genes encoding for cell wall and membrane biosynthetic enzymes showed transcript abundance patterns consistent with completion of cell maturation, and also of cell wall and membrane modifications potentially enabling cells to withstand the harsh conditions of winter. Several differentially expressed genes were identified that encoded putative regulators of cambial activity, cell development, and of the photoperiodic pathway. Reconfiguration of carbon allocation figured centrally in the tree’s overwintering preparations. For example, genes associated with carbon-based defenses such as terpenoids were downregulated, while many genes associated with proteinbased defenses and other stress mitigation mechanisms were upregulated. Several of these correspond to proteins that were accumulated during the growth-to-dormancy transition, emphasizing the importance of stress protection in the tree’s adaptive response to overwintering.
Overall design Two year old white spruce (Picea glauca [Moench] Voss) seedlings were used for all experiments, which were conducted as described by El Kayal et al. (2011). Using a complete randomized block design, seedlings were grown in growth chambers under long days (LD; 16h day / 8h night, 20°C, 50 to 60% RH) for 8-10 weeks. Shortly before seedlings were to begin bud formation, the photoperiod was changed to short days (SD; 8h day / 16h night, 20°C, 50 to 60% RH) to induce rapid and synchronous bud formation. This was designated Day 0. Lignified whole stems representing the current year’s growth (microarrays, microscopy) or previous year’s growth (protein analyses) were sampled at 0, 3, 7, 14, 28 and 70 d (10 wk) SD, and immediately frozen in liquid nitrogen. Remaining plants were maintained in SD for an additional 8-15 wk, and then transferred to low temperatures (LT, 2 - 4°C) for 3 to 4 weeks with continuing SD prior to harvest. These are referred to as LT samples.
Contributor(s) Galindo González LM, El Kayal W, Ju CJ, Allen CC, King-Jones S, Cooke JE
Citation(s) 21988609
Submission date Aug 01, 2011
Last update date Mar 23, 2012
Contact name Chelsea Ju
Organization name University of Alberta
Department Biological Sciences
Lab Dr. Janice Cooke
Street address CW 405, Biological Sciences Centre
City Edmonton
State/province Alberta
ZIP/Postal code T6G 2E9
Country Canada
Platforms (1)
GPL10754 Arborea spruce 11K cDNA microarray
Samples (4)
GSM769885 SD Day 3 vs SD Day 0
GSM769886 SD Day 7 vs SD Day 0
GSM769887 SD Day 14 vs SD Day 0
BioProject PRJNA144705

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Supplementary file Size Download File type/resource
GSE31090_RAW.tar 48.8 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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