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Series GSE31068 Query DataSets for GSE31068
Status Public on Dec 31, 2011
Title Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture
Platform organism Streptomyces coelicolor A3(2)
Sample organism Streptomyces coelicolor
Experiment type Expression profiling by array
Summary Bacteria in the genus Streptomyces are soil dwelling oligotrophs and important producers of secondary metabolites. Previously we showed that global mRNA expression was subject to a series of metabolic and regulatory switches during the life time of a fermentor batch culture of S. coelicolor M145. Here we analyse the proteome from eight time points from the same fermentor culture and, as phosphate availability is an important regulator of secondary metabolite production, compare this to the proteome of a similar time course from an S. coelicolor mutant, INB201 (ΔphoP), defective in the control of phosphate utilisation. The proteomes provide a detailed view of enzymes involved in central carbon and nitrogen metabolism. Trends in protein expression over the time courses were deduced from a protein abundance index which also revealed the importance of stress pathway proteins in both cultures. As expected the ΔphoP mutant was deficient in expression of PhoP-dependent genes and several putatively compensatory metabolic and regulatory pathways for phosphate scavenging were detected. Notably there is a succession of switches that co-ordinately induce the production of enzymes for five different secondary metabolite biosynthesis pathways over the course of the batch cultures and these were not confined to the stationary phase.
 
Overall design 36 samples, no replicates; one hour resolution from 23-36h and 41-48h; half hour resolution from 36-41h; two hour resolution 48-60h; sample missing for 34 h
 
Contributor(s) Nieselt K, Wentzel A, Smith MC, Consortium S
Citation(s) 22147733
Submission date Jul 31, 2011
Last update date Jul 31, 2013
Contact name Kay Nieselt
E-mail nieselt@informatik.uni-tuebingen.de
Phone (+49) 70712978981
Organization name Center for Bioinformatics Tuebingen
Department Department for Information and Cognitive Sciences
Street address Sand 14
City Tübingen
State/province DE
ZIP/Postal code 72138
Country Germany
 
Platforms (1)
GPL9417 Streptomyces coelicolor Affymetrix array
Samples (36)
GSM769467 phoP-F134-F335-23 h
GSM769468 phoP-F134-F335-24 h
GSM769469 phoP-F134-F335-25 h
Relations
BioProject PRJNA144841

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31068_RAW.tar 43.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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