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| Status |
Public on Jul 25, 2011 |
| Title |
Neutrophil expression array |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Primary tumors have been shown to prepare distal organs for later colonization of metastatic cells by stimulating organ-specific infiltration of bone marrow-derived cells. Here we demonstrate that neutrophils accumulate in the lung prior to the arrival of metastatic cells in mouse models of breast cancer. Tumor-entrained neutrophils (TENs) inhibit metastatic seeding in the lungs by generating H2O2, and tumor-secreted CCL2 is a critical mediator of optimal anti-metastatic entrainment of G-CSF-stimulated neutrophils. TENs are present in the peripheral blood of breast cancer patients prior to surgical resection but not in healthy individuals. Thus, while tumor-secreted factors contribute to tumor progression at the primary site, they concomitantly induce a neutrophil-mediated inhibitory process at the metastatic site.
This experiment was designed to compare the RNA expression profile in resting (control) neutrophils vs. that of TENs (neutrophils purified from tumor-bearing mice). In addition, we also compared the expression profiles of cultured neutrophils in vitro and treated with CCL2, CCL5 and GCSF.
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| Overall design |
Circulating neutrophils were purified from control and 4T1 tumor-bearing mice and flash frozen. For in vitro cultures, circulating neutrophils were purified from control mice, cultured in the presence of chemokines for 2 hours and flash frozen.
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| Contributor(s) |
Granot Z, Benezra R |
| Citation(s) |
21907922 |
| Submission date |
Jul 22, 2011 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Yupu Liang |
| E-mail(s) |
liangy@rockefeller.edu
|
| Organization name |
The Rockefeller University
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE30888 |
Tumor-entrained neutrophils inhibit seeding in the pre-metastatic lung |
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| Relations |
| BioProject |
PRJNA154587 |
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