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| Status |
Public on Aug 22, 2012 |
| Title |
Gene expression changes in the septum: possible implications for microRNAs in sculpting the maternal brain. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The transition from the non-maternal to the maternal state is characterized by a variety of CNS alterations that support the care of offspring. The septum (including lateral and medial portions) is a brain region previously linked to various emotional and motivational processes, including maternal care. In this study, we used microarrays (PLIER algorithm) to examine gene expression changes in the septum of postpartum mice and employed gene set enrichment analysis (GSEA) to identify possible regulators of altered gene expression. Genes of interest identified as differentially regulated with microarray analysis were validated with quantitative real-time PCR. We found that fatty acid binding protein 7 (Fabp7) and galanin (Gal) were downregulated, whereas insulin-like growth factor binding protein 3 (Igfbp3) was upregulated in postpartum mice compared to virgin females. These genes were previously found to be differentially regulated in other brain regions during lactation. We also identified altered expression of novel genes not previously linked to maternal behavior, but that could play a role in postpartum processes, including glutamate-ammonia ligase (Glul) and somatostatin receptor 1 (Sstr1) (both upregulated in postpartum). Genes implicated in metabolism, cell differentiation, or proliferation also exhibited altered expression. Unexpectedly, enrichment analysis revealed a high number of microRNAs, transcription factors, or conserved binding sites (177 with corrected P-value <0.05) that were significantly linked to maternal upregulated genes, while none were linked to downregulated genes. MicroRNAs have been linked to placenta and mammary gland development, but this is the first indication they may also play a key role in sculpting the maternal brain. Together, this study provides new insights into genes (along with possible mechanisms for their regulation) that are involved in septum-mediated adaptations during the postpartum period.
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| Overall design |
In total, 12 total RNA samples derived from microdissected lateral septum were used for microarray analysis: 6 samples from virgin mice and 6 samples from age-matched lactating maternal mice. All samples are biological replicates.
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| Contributor(s) |
Zhao C, Saul MC, Driessen TM, Gammie SC |
| Citation(s) |
22701680 |
| Submission date |
Jul 21, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Michael C. Saul |
| E-mail(s) |
michael.saul@jax.org
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| Organization name |
The Jackson Laboratory
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| Lab |
Chesler Lab
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| Street address |
600 Main St
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| City |
Bar Harbor |
| State/province |
ME |
| ZIP/Postal code |
04609 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA144171 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30836_RAW.tar |
53.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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