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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 02, 2012 |
| Title |
Gene expression data from human metastatic melanoma tumors that may predict response to RAF265 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To determine how mutation of BRAF affected the response to RAF265, we utilized a tumor orthotopic implant model of early passage melanoma tumors in nude mice from a series of 17 patients with advanced metastatic Tumor growth was compared between RAF265 treatment (40 mg/kg, QD) and diluent control groups. The melanoma associated gene mutation profile and global gene expression profile were determined on these human melanoma samples by SNaPshot and Affymetrix Human Gene ST 1.0 Array, respectively. Tumors were evaluated for growth response to RAF265 in an orthotopic implant model using nude mice. Comparisons were made between gene expression profiles of responders and non-responders, BRAF mutant and BRAF wild type tumors. Analysis of the microarray data revealed responders exhibited enriched expression of genes involved in cell cycle, apoptosis, cell-cell adhesion and initiation of epithelial/mesenchymal transition. It is concluded that RAF265 significantly inhibits the growth of a sub-population of V600E mutant and wild type BRAF human melanoma tumors in vivo and the gene expression profile of this subset of tumors that may predict response to RAF265.
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| Overall design |
RNA from 17 human metastatic melanoma tumors was extracted and hybridized on Affymetrix microarrays.
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| Contributor(s) |
Richmond A, Su Y, Levy S, Jiang A, Lu P, Shyr Y, Sosman JA, Kelley MC, Dahlman KB, Stuart DD, Splittgerber RC, Kantrow S, Short SP, Sobolik-Delmaire T, Zaja-Milatovic S |
| Citation(s) |
22351689 |
| Submission date |
Jul 20, 2011 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Pengcheng Lu |
| E-mail(s) |
pengcheng.lu@vanderbilt.edu
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| Organization name |
Vanderbilt University
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| Street address |
2200 Pierce Avenue
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| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (42)
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| GSM764225 |
Melanoma Tumor V28, biological rep1 |
| GSM764226 |
Melanoma Tumor V28, biological rep2 |
| GSM764227 |
Melanoma Tumor V28, biological rep3 |
| GSM764228 |
Melanoma Tumor V35, biological rep1 |
| GSM764229 |
Melanoma Tumor V35, biological rep2 |
| GSM764230 |
Melanoma Tumor V35, biological rep3 |
| GSM764231 |
Melanoma Tumor V19, biological rep1 |
| GSM764232 |
Melanoma Tumor V19, biological rep2 |
| GSM764233 |
Melanoma Tumor V33, biological rep1 |
| GSM764234 |
Melanoma Tumor V33, biological rep2 |
| GSM764235 |
Melanoma Tumor V33, biological rep3 |
| GSM764236 |
Melanoma Tumor V34, biological rep1 |
| GSM764237 |
Melanoma Tumor V34, biological rep2 |
| GSM764238 |
Melanoma Tumor V34, biological rep3 |
| GSM764239 |
Melanoma Tumor V32, biological rep1 |
| GSM764240 |
Melanoma Tumor V32, biological rep2 |
| GSM764241 |
Melanoma Tumor V32, biological rep3 |
| GSM764242 |
Melanoma Tumor V25, biological rep1 |
| GSM764243 |
Melanoma Tumor V25, biological rep2 |
| GSM764244 |
Melanoma Tumor V24, biological rep1 |
| GSM764245 |
Melanoma Tumor V24, biological rep2 |
| GSM764246 |
Melanoma Tumor V27, biological rep1 |
| GSM764247 |
Melanoma Tumor V27, biological rep2 |
| GSM764248 |
Melanoma Tumor V27, biological rep3 |
| GSM764249 |
Melanoma Tumor V05, biological rep1 |
| GSM764250 |
Melanoma Tumor V05, biological rep2 |
| GSM764251 |
Melanoma Tumor V30, biological rep1 |
| GSM764252 |
Melanoma Tumor V30, biological rep2 |
| GSM764253 |
Melanoma Tumor V30, biological rep3 |
| GSM764254 |
Melanoma Tumor V12, biological rep1 |
| GSM764255 |
Melanoma Tumor V12, biological rep2 |
| GSM764256 |
Melanoma Tumor V23, biological rep1 |
| GSM764257 |
Melanoma Tumor V23, biological rep2 |
| GSM764258 |
Melanoma Tumor V18, biological rep1 |
| GSM764259 |
Melanoma Tumor V18, biological rep2 |
| GSM764260 |
Melanoma Tumor V29, biological rep1 |
| GSM764261 |
Melanoma Tumor V29, biological rep2 |
| GSM764262 |
Melanoma Tumor V29, biological rep3 |
| GSM764263 |
Melanoma Tumor V13, biological rep1 |
| GSM764264 |
Melanoma Tumor V13, biological rep2 |
| GSM764265 |
Melanoma Tumor V15, biological rep1 |
| GSM764266 |
Melanoma Tumor V15, biological rep2 |
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| Relations |
| BioProject |
PRJNA144217 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE30812_RAW.tar |
172.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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