Expression profiling by high throughput sequencing Other
Summary
we introduce a new perturbational scRNA-seq dataset that includes six immortalized lines (A549, A375, H1AE, HEK293T, HEP2G, and PC3) and four primary lines: CD8+ T-cells (CD8+), CD34+ hematopoietic progenitors (CD34+), primary adipocytes (PAD), and bronchial epithelial cells (HBEC). 104 compounds, including 88 from CMap, were tested across the 10 cell types in duplicate with library-matched controls, resulting in 1,737 scRNA-seq samples comprising a total of 1.26 M cells.
Overall design
Hashed single-cell RNA sequencing of cancer and primary cell lines, performed 24 hours after perturbation by 104 small molecules, with library-matched controls. Each plate repesents a different cell line. Cell lines are profiled in at least two distinct replicate plates each, per compound.
Less...
More...